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Fragment Libraries			Alanine Scanning			DNA Interface Scan
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Job 79433 [SSGCID - LeinA.19528.a] [2017-11-20] [D-172-16-30-137.dhcp4.x.x] [Structure] [Complete] J-binding protein 2 (JBP2) - BATCH:79431

Features and Secondary Structure

1 . 10 . 20 . 30 . 40 . 50 . 60 . 70 . 80 . 90 . 100 . 110 . 120 . 130 . 140 . 150 . 160 . 170 . 180 . 190 . 200 . 210 . 220 . 230 . 240 . 250 . 260 . 270 . 280 . 290 . 300 . 310 . 320 . 330 . 340 . 350 . 360 . 370 . 380 . 390 . 400 . 410 . 420 . 430 . 440 . 450 . 460 . 470 . 480 . 490 . 500 . 510 . 520 . 530 . 540 . 550 . 560 . 570 . 580 . 590 . 600 . 610 . 620 . 630 . 640 . 650 . 660 . 670 . 680 . 690 . 700 . 710 . 720 . 730 . 740 . 750 . 760 . 770 . 780 . 790 . 800 . 810 . 820 . 830 . 840 . 850 . 860 . 870 . 880 . 890 . 900 . 910 . 920 . 930 . 940 . 950 . 960 . 970 . 980 . 990 . 1000 . 1010 . 1020 . 1030 . 1040 . 1050 . 1060 . 1070 . 1080 . 1090

MLNGLTRVSTSSELESILDIVQSSGEIAVVFTSPSIGDLETITSETQRRQLRIAGIPRGGYTILPAIPLYDDELLQMCERYTSANEYEKAEMRNSLYMREYPLFTYSIRHQRALFHPADYVSRILQFCSYYVQAPDADVLPLQDKSPFLHISPIKEICKHLRLIARGTPVAPDDSESPVPEQLRLHAESDAEKLAAERATAMSIATSSGGASETEQPSLFSGVAPSALFQKGAVEEVDKDAEDTMEDLTGEETVDAVHSFQAEYLTLDGFELVTKASIYYDREGEGQRVVAVYIPGGVPEDTCRAAAAVLEPAATKKNLRAPTNGGLPPDTGIVGYYDYLTNPTQHKCRETEYSRRNWGLLAQSEPLLKHLDKLYSQLAPMHHHLQRVAIPSQYQLCGTVFSTITVNRNFRTAVHTDKGDFRSGLGVLSVINGEFEGCHLAIKRLKKAFQLKVGDVLLFDTSLEHGNTEVVNPEIHWQRTSVVCYLRTGLMSSVCEMERRKHLNRLILLQLLNTEVRNTTVNINGADSSLPPLFVPTRLASHLAPVQLAALGFIVERTEKQSGCVVAMTMGLGKTLVALTLCFSQLHLAPQADILILTPKPIISHWVDEKNKWGMHGLHFPHFVASDGLNSLEFEQQLLEYERQRNNEKPKLGHIFVINGEYLAGFLRRFKRFTPLLMIVDEGHRVAAKGNKLTESLDRLRCNLRIVLSGTPLQNDASELYRLVGWVNKGVSRVLPPKRFQELANDINQFVEGDDGAFYNAVMAQEYIQDWMRGFVFREMENDLPPLHDYLLICGSSDVQREYEEKLGLTETAMTALKATEHRPHHLSTHPACYLAFISDSYQSMVSGWTVRAQSNTSRPRVSQLEEIDTMRLEQYVQLVENEQLDAFIDLSGKMRVLVDIVLRVQARKEKLIIFSLYVGSQDLIHRTLTALRVCTFTVRGRDSQDRRRRAMQEFSENKDLIVLVLSTKIAAYGLDFTAANHVVLFDSWWNPQVDAQAIARAYRRNQRKPVTVYRLISATENKFVLRSQTRKIALFKCILHERTSRQALPDELEDCAANEKDEERRIFWAKLKTTSLAGDSRALLNVYRYQESVRESE

tmhmm (0)
------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

low complexity (2%)
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------XXXXXXXXXX-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------XXXXXXXXXXX----------------------------------------------------------------------------------------------------------------------------------------------------

coiled-coils (0%)
------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

disordered (21%)
XXXXXXXX-------------------------------------------------------------------------------------------------------------------------------------------------------------------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX------XXX-XXXXX-----------------------------------------------------------------------------------------X---------------------------------------------------------------------------------------------------------------------------------------------------------------------------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX--------------------------------------------------------------------------------------------------------XXXXXXXXXX----------------------------------------------------------------------------------------------XXXXXXXXXXXXXXXXXXXXXXXXX-----------------------------------------------XXXXXXXXXXXXX----------------XXXXXXXXXXXXXXXXXXX------------------------------------------------------------------------------------XXXXXXX-------------------------------------------------------------------------------------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX------X-XXXXXX

psipred
------EEEHHHHHHHHHHHHHH----EEEE-------HHHHHHHHHH---EEE------EEE--------HHHHHHHHHHHH---HHHHHHHHHHHHHH----------------HHHHHHHHHHHHHHH----HHHHH------------HHHHHHHHHHHHHH-------HHH---------HHH--HHHHHH---HHHHH-------------------------------------------------------HHHHHH---------HHHHHH-------EEEEEE---HHHHHHHHHHHHHHH-----HHHHHHH------------HHH----HHHHHHHHHHHHHHHHHHHH---HHHHHHHHHHHHHHHHHHHHHHHH--HHHHH------EEEE-------EE-----EE---------HHHHHHHHHHHHHH-HHH------EEE--HHHHHHHHHHH-HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH-HHHH--HHHH-------------HHH-----HHHHHHHHHHHHHHH----EEEE------HHHHHHHHHHHHHHH-----EEEEE-HHHHHHHHHHHHHH----EEEEEE-------HHHHHHHHHHHHHHH---------EEE--HHHHHHHHHHH------EEEEE-HHH---HHHHHHHHHHH--EEEEEE---------HHHHHHHHHHH-------HHHHHHHHH--HHHH---HHHHHHHHHHHHHHHHHHH---EEHHHHH------EEEEEEE---HHHHHHHHHHHHHHHHHHHHHHHHHHH---HHHHHHHHHHH---------HHHHHHHHHH---HHHHH--------HHH------HHHHHHH---HHHHHHHHHHHHHHH---EEEEEE-HHHHHHHHHHHHHH----EEEE-----HHHHHHHHHH--------EEEEE--------------EEEE------HHHHHHHHHHH-------EEEEEE-----HHHHHHHHHHHHHHHHHHHH---------HHHHHHHHH--HHHHHHHHHH----------HHHHHHHHH--HHHH---

# SignalP-4.0 gram+ predictions
# Measure  Position  Value  Cutoff  signal peptide?
  max. C    24	    0.141
  max. Y    24	    0.150
  max. S    22	    0.235
  mean S     1-23   0.164
       D     1-23   0.155    0.450  NO
Name=tmp_signalp_seq	SP='NO' D=0.155 D-cutoff=0.450 Networks=SignalP-TM

Domain Repeats Prediction

Boundary STD (+/-) Consensus

-- -- --

Ginzu Domain Prediction 1

Domain Span Source Reference Parent Parent Span Confidence Annotations

1-204 alignment 2o5cA_201 1-634 0.148400 ISOMERASE/DNA

205-269 alignment 4l92B_201 1-391 0.0966 --

270-489 alignment 4lt5A_201 1-265 0.711000 --

490-1098 alignment 1z3iX_302 1-644 0.485200 Recombination/DNA binding

Ginzu Domain Prediction 2

Domain Span Source Reference Parent Parent Span Confidence Annotations

1-204 msa 2o5cA_201 1-634 0.148400 --

205-269 cutpref -- -- 0.000000 --

270-489 alignment 4lt5A_201 1-265 0.711000 --

490-1098 alignment 1z3iX_302 1-644 0.485200 Recombination/DNA binding

PSI-BLAST Sequence Hits (Top 20 by Identity) ALL DATA

TaxID Species Alignments PSI-Blast Data (alignment with lowest E value)

E value Query Span Sbjct Span Bit Score Identity HSP Length Accession Description

0 UNIDENTIFIED 47 (1.18%) 0.0 1-1098 1-1098 1157 100 1098 sp|A4HVU6.2|JBP2_LEIIN RecName: Full=Bifunctional helicase and thymine dioxygenase ...

435258 Leishmania infantum JPCM5 10 (0.25%) 0.0 77-1098 1-1022 1039 100 1022 ref|XP_001464187.1| putative j-binding protein [Leishmania infantum JPCM5] emb|C...

5661 Leishmania donovani 2 (0.05%) 0.0 77-1098 1-1022 1038 100 1022 ref|XP_003859376.1| j-binding protein, putative [Leishmania donovani] emb|CBZ326...

347515 Leishmania major strain Friedlin 11 (0.27%) 0.0 77-1098 1-1022 1012 97 1022 ref|XP_001687603.1| putative j-binding protein [Leishmania major strain Friedlin...

929439 Leishmania mexicana MHOM/GT/2001/U1103 2 (0.05%) 0.0 77-1098 1-1022 996 96 1022 ref|XP_003873375.1| putative j-binding protein [Leishmania mexicana MHOM/GT/2001...

5689 Leishmania tarentolae 1 (0.03%) 0.0 1-1098 1-1098 1112 95 1098 sp|B6EU02.1|JBP2_LEITA RecName: Full=Bifunctional helicase and thymine dioxygenase ...

420245 Leishmania braziliensis MHOM/BR/75/M2904 9 (0.22%) 0.0 1-1098 1-1098 1106 90 1098 ref|XP_001562198.1| putative j-binding protein [Leishmania braziliensis MHOM/BR/...

134006 Phytomonas sp. isolate EM1 1 (0.03%) 0.0 1-1098 1-1078 915 53 1102 emb|CCW64034.1| unnamed protein product [Phytomonas sp. isolate EM1]

28005 Strigomonas culicis 4 (0.1%) 0.0 12-1098 6-1077 940 51 1094 gb|EPY21080.1| DNA repair and recombination protein RAD54B [Strigomonas cul...

134013 Phytomonas sp. isolate Hart1 1 (0.03%) 0.0 5-1098 2-1080 924 51 1097 emb|CCW69781.1| unnamed protein product [Phytomonas sp. isolate Hart1]

59799 Angomonas deanei 1 (0.03%) 1E-144 319-1075 1-755 520 50 762 gb|EPY28945.1| DNA repair and recombination protein RAD54B [Angomonas deane...

5693 Trypanosoma cruzi 3 (0.07%) 0.0 12-1097 10-1083 933 48 1101 gb|EKF98730.1| SNF2 DNA repair protein, putative [Trypanosoma cruzi]

85056 Trypanosoma cruzi marinkellei 1 (0.03%) 0.0 12-1097 9-1082 915 48 1101 gb|EKF28363.1| SNF2 DNA repair protein, putative [Trypanosoma cruzi marinke...

679716 Trypanosoma brucei gambiense DAL972 1 (0.03%) 0.0 10-1097 8-1074 870 43 1093 emb|CBH12615.1| SNF2 DNA repair protein, putative [Trypanosoma brucei gambie...

999953 Trypanosoma brucei brucei strain 927/4 GUTat10.1 4 (0.1%) 0.0 10-1097 8-1074 869 43 1093 ref|XP_846088.1| SNF2 DNA repair protein [Trypanosoma brucei brucei strain 92...

1055687 Trypanosoma vivax Y486 2 (0.05%) 1E-155 1-609 1-592 556 43 628 emb|CCC49143.1| putative SNF2 DNA repair protein, fragment [Trypanosoma viva...

1163396 Pseudomonas aeruginosa CI27 1 (0.03%) 1E-100 532-1052 181-649 374 23 527 ref|YP_792483.1| helicase [Pseudomonas aeruginosa UCBPP-PA14] ref|WP_00314091...

287 Pseudomonas aeruginosa 1 (0.03%) 1E-100 532-1052 291-759 374 23 523 ref|WP_009875983.1| helicase [Pseudomonas aeruginosa] gb|ERU69143.1| helicase [P...

350704 Pseudomonas aeruginosa C3719 1 (0.03%) 1E-100 532-1052 181-649 374 23 523 ref|WP_004348251.1| helicase [Pseudomonas aeruginosa] gb|EAZ56599.1| hypothetica...

381754 Pseudomonas aeruginosa PA7 2 (0.05%) 1E-100 526-1052 285-759 373 23 533 ref|YP_001350061.1| helicase [Pseudomonas aeruginosa PA7] ref|WP_012077012.1| he...