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Fragment Libraries			Alanine Scanning			DNA Interface Scan
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Job 79436 [SSGCID - LemeA.19528.a] [2017-11-20] [D-172-16-30-137.dhcp4.x.x] [Structure] [Complete] J-binding protein 2 (JBP2) - BATCH:79431

Features and Secondary Structure

1 . 10 . 20 . 30 . 40 . 50 . 60 . 70 . 80 . 90 . 100 . 110 . 120 . 130 . 140 . 150 . 160 . 170 . 180 . 190 . 200 . 210 . 220 . 230 . 240 . 250 . 260 . 270 . 280 . 290 . 300 . 310 . 320 . 330 . 340 . 350 . 360 . 370 . 380 . 390 . 400 . 410 . 420 . 430 . 440 . 450 . 460 . 470 . 480 . 490 . 500 . 510 . 520 . 530 . 540 . 550 . 560 . 570 . 580 . 590 . 600 . 610 . 620 . 630 . 640 . 650 . 660 . 670 . 680 . 690 . 700 . 710 . 720 . 730 . 740 . 750 . 760 . 770 . 780 . 790 . 800 . 810 . 820 . 830 . 840 . 850 . 860 . 870 . 880 . 890 . 900 . 910 . 920 . 930 . 940 . 950 . 960 . 970 . 980 . 990 . 1000 . 1010 . 1020 . 1030 . 1040 . 1050 . 1060 . 1070 . 1080 . 1090

MLNGLTRVSTSSELESILDIVQSSGEIAVVFTSPSIGDLETITSETQRRQLRIAGIPRGGYTILPAIPLYDDELLQMCERYTAANEYEKAEMRNGLYMREYPLFAYSMRHQRALFHPADYVSRILQFCSYYVQVPDTDVLSLQDRSPFLHISPIKEICTRLRLIARGTPVAPDDSESPVPEQLRFHAESDVEKLATECSTAMTIAASSGGASETEQLPLFSGVAPSALFQKDAVEEVDKDAEEAMEDLTGEETVDAVHSFQPEYLTLDGFELVTKASIFYDREGEGQRVVAVYIPGGVPEDTCRAAAAVLEPAATKKNLRAPTNGGLPPDTGIVGYYDYLTNPTQHKCRETEFSRRNWGLLAQSEQLLKHLDKLYSQLAPMHHHLQRVAIPSQYQLCGTVFSTITVNRNFRTAVHTDKGDFRSGLGVLSVINGEFEGCHLAIKRLKKAFQLKVGDVLLFDTSLEHGNTEVINPEIHWQRTSVVCYLRTGLMSSVCEMERRKHLNRLILQQLLNTEVRHTTVNINGADSSLPPLFVPTRLASHLAPVQLSALGFIVERAEKQSGCVVAMTMGLGKTLVALTLCFSQLHLAPQADILILTPKPIISHWVDEKNKWGMHGLHFPHFVASDGLNSLEFEQQLLEYERQKNNEKPKLGHIFVINGEYLAGFLRRFKRFTPLLMIVDEGHRVAAKGNKLTESLDRLRCNLRIVLSGTPLQNDASELYRLVGWVNKGVSRVLPPKRFQELANDINQFVEGDDGAFYNAVMAQEYIQDWMRGFVFREMENDLPPLHDYLLICGSSDVQLEYEEKLGLTETAMAALKATEHRPHHLSTHPACYLAFISDSYQSMVSGWTVRAQPNTSRSRVSQLEEIDTMRLEQYVQLVENEQLDAFIDLSGKMRVLVDIVLRVQARKEKLIIFSLYVGSQDLIHRTLTALRVCTFTVRGRDSQDRRRRAMQEFSENKDLIVLVLSTKIAAYGLDFTAANHVVLFDSWWNPQVDAQAIARAYRRNQRKPVTVYRLISATENKFVLRSQTRKIALFKCILHERTSRQALPDELEDCAANEKDEERKNFWAKLKTASLAGGTRALLNVYRYQESVRESE

tmhmm (0)
------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

low complexity (3%)
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------XXXXXXXXXXXXXXXXX--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------XXXXXXXXXXX----------------------------------------------------------------------------------------------------------------------------------------------------

coiled-coils (2%)
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------XXXXXXXXXXXXXXXXXXXXX-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

disordered (22%)
XXXXXXXX----------------------------------------------------------------------------------------------------------------------------------------------------------------------XXXXXXXXXXXXXXX------XXXXXXXXXXXXXXXXXXXXXX-XX----XXXXXXXX-----------------------------------------------------------------------------------------XXX----------------------------------------------------------------------------------------------------------------------------------------------------------------------------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX---------------------------------------------------------------------------------------------------XXXXXXXXXXXXXXXXXX---------------------------------------------------------------------------------------------XXXXXXXXXXXXXXXXXXXXXXXX----------------------------------------------XXXXXXXXXXXXXXXXXX------------------XXXXXXXXXXXXXXXXXXXXX-------------------------------------------------------------------------------XXXXXXXXX-----------------------------------------------------------------------------------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX---XXXXXXXXX

psipred
------EEEHHHHHHHHHHHHHH----EEEE------HHHHHHHHHHH---EEE------EEE--------HHHHHHHHHHH---HHHHHHHH--HHHH----------HHHH---HHHHHHHHHHHHHHH----HHHHHHHHHH-HHH---HHHHHHHHHHHHHH--------------------HHHHHHHHHHH-HHHHH---------HHHH---------HHHHHH----HHHH----------HHHHHH------HHHHH------------EE-------EEEEEE---HHHHHHHHHHHHH------HHHHHHHH-------------------HHHHHHHHHHHHHH--HHH--HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH--EE--EEEE-------EE-----EEEE-------HHHHHHHHHHHHH----EE-----EEE--HHHHHHHHHHH-HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH-HHHHHHHHHH-------------HHH-----HHHHHHHHHHHHHHH----EEEE-------HHHHHHHHHHHHHH-----EEEEE-HHHHHHHHHHHHHH----EEEEEEE------HHHHHHHHHHHHHHHH--------EEE--HHHHHHHHHHHH-----EEEE--HHH-----HHHHHHHH-EEEEEEEE---------HHHHHHHHHHH--------HHHHHHHH--HHHH-----HHHHHHHHHHHHHHHHHH--EEHHHHH------EEEEEEE---HHHHHHHHHHHHHHHHHHHHHH-----HHHHHHHHHHHHHHHH--HHHHHHHHH----HHHHHHHHHHH--------------------------HHHHHHHHHHHHHHH----EEEEE-HHHHHHHHHHHHHH----EEEE-----HHHHHHHHHHH-------EEEEE--------------EEEEE-----HHHHHHHHHHHHH------EEEEE-----HHHHHHHHHHHHHHHHHHHH---------HHHHHHHHH----HHHHHHHHHHHHHHHH---HHH--------HHH---

# SignalP-4.0 gram+ predictions
# Measure  Position  Value  Cutoff  signal peptide?
  max. C    24	    0.141
  max. Y    24	    0.150
  max. S    22	    0.235
  mean S     1-23   0.164
       D     1-23   0.155    0.450  NO
Name=tmp_signalp_seq	SP='NO' D=0.155 D-cutoff=0.450 Networks=SignalP-TM

Domain Repeats Prediction

Boundary STD (+/-) Consensus

-- -- --

Ginzu Domain Prediction 1

Domain Span Source Reference Parent Parent Span Confidence Annotations

1-203 alignment 2l82A_101 1-162 0.152800 DE NOVO PROTEIN

204-332 alignment 3it4B_201 1-202 0.103400 TRANSFERASE

333-444 alignment 5d9yA_301 1-413 0.696900 --

445-530 alignment 2a1xA_201 1-252 0.237300 OXIDOREDUCTASE

531-1098 alignment 5x0xO_301 1-583 0.533500 --

Ginzu Domain Prediction 2

Domain Span Source Reference Parent Parent Span Confidence Annotations

1-203 msa 2l82A_101 1-162 0.152800 --

204-332 msa 3it4B_201 1-202 0.103400 --

333-444 alignment 5d9yA_301 1-413 0.696900 --

445-530 msa 2a1xA_201 1-252 0.237300 --

531-1098 alignment 5x0xO_301 1-583 0.533500 --

PSI-BLAST Sequence Hits (Top 20 by Identity) ALL DATA

TaxID Species Alignments PSI-Blast Data (alignment with lowest E value)

E value Query Span Sbjct Span Bit Score Identity HSP Length Accession Description

929439 Leishmania mexicana MHOM/GT/2001/U1103 2 (0.05%) 0.0 77-1098 1-1022 1088 100 1022 ref|XP_003873375.1| putative j-binding protein [Leishmania mexicana MHOM/GT/2001...

0 UNIDENTIFIED 30 (0.75%) 0.0 1-1098 1-1098 1187 96 1098 sp|Q4QFY1.2|JBP2_LEIMA RecName: Full=Bifunctional helicase and thymine dioxygenase ...

347515 Leishmania major strain Friedlin 7 (0.18%) 0.0 77-1098 1-1022 1067 96 1022 ref|XP_001687603.1| putative j-binding protein [Leishmania major strain Friedlin...

435258 Leishmania infantum JPCM5 6 (0.15%) 0.0 77-1098 1-1022 1048 96 1022 ref|XP_001464187.1| putative j-binding protein [Leishmania infantum JPCM5] emb|C...

5661 Leishmania donovani 2 (0.05%) 0.0 77-1098 1-1022 1048 96 1022 ref|XP_003859376.1| j-binding protein, putative [Leishmania donovani] emb|CBZ326...

5689 Leishmania tarentolae 1 (0.03%) 0.0 1-1098 1-1098 1164 94 1098 sp|B6EU02.1|JBP2_LEITA RecName: Full=Bifunctional helicase and thymine dioxygenase ...

420245 Leishmania braziliensis MHOM/BR/75/M2904 6 (0.15%) 0.0 1-1098 1-1098 1158 90 1098 ref|XP_001562198.1| putative j-binding protein [Leishmania braziliensis MHOM/BR/...

134006 Phytomonas sp. isolate EM1 1 (0.03%) 0.0 1-1098 1-1078 973 54 1104 emb|CCW64034.1| unnamed protein product [Phytomonas sp. isolate EM1]

28005 Strigomonas culicis 4 (0.1%) 0.0 12-1098 6-1077 982 52 1095 gb|EPY21080.1| DNA repair and recombination protein RAD54B [Strigomonas cul...

134013 Phytomonas sp. isolate Hart1 1 (0.03%) 0.0 5-1098 2-1080 963 51 1098 emb|CCW69781.1| unnamed protein product [Phytomonas sp. isolate Hart1]

59799 Angomonas deanei 1 (0.03%) 1E-147 319-1077 1-757 531 49 764 gb|EPY28945.1| DNA repair and recombination protein RAD54B [Angomonas deane...

5693 Trypanosoma cruzi 3 (0.08%) 0.0 12-1095 10-1081 988 48 1099 gb|EKF98730.1| SNF2 DNA repair protein, putative [Trypanosoma cruzi]

85056 Trypanosoma cruzi marinkellei 1 (0.03%) 0.0 12-1095 9-1080 979 48 1099 gb|EKF28363.1| SNF2 DNA repair protein, putative [Trypanosoma cruzi marinke...

679716 Trypanosoma brucei gambiense DAL972 1 (0.03%) 0.0 10-1095 8-1072 927 43 1091 emb|CBH12615.1| SNF2 DNA repair protein, putative [Trypanosoma brucei gambie...

999953 Trypanosoma brucei brucei strain 927/4 GUTat10.1 3 (0.08%) 0.0 10-1095 8-1072 926 43 1091 ref|XP_846088.1| SNF2 DNA repair protein [Trypanosoma brucei brucei strain 92...

1055687 Trypanosoma vivax Y486 1 (0.03%) 1E-163 1-611 1-594 582 42 629 emb|CCC49143.1| putative SNF2 DNA repair protein, fragment [Trypanosoma viva...

452589 Trichoderma atroviride IMI 206040 3 (0.08%) 1E-117 525-1062 195-733 431 23 566 gb|EHK43646.1| hypothetical protein TRIATDRAFT_319009 [Trichoderma atroviri...

5141 Neurospora crassa 2 (0.05%) 1E-117 527-1061 205-740 431 23 563 ref|XP_959470.1| DNA repair and recombination protein RAD54 [Neurospora crass...

242507 Magnaporthe oryzae 70-15 2 (0.05%) 1E-117 456-1062 145-734 429 23 635 ref|XP_003716366.1| DNA repair protein rhp54 [Magnaporthe oryzae 70-15] dbj|BAC1...

350704 Pseudomonas aeruginosa C3719 1 (0.03%) 1E-102 532-1052 181-649 381 23 527 ref|WP_004348251.1| helicase [Pseudomonas aeruginosa] gb|EAZ56599.1| hypothetica...