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Fragment Libraries			Alanine Scanning			DNA Interface Scan
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Job 81211 [SSGCID - MygeA.20084.a] [2018-02-01] [D-172-16-30-137.dhcp4.x.x] [Structure] [Complete] Cytadherence high molecular weight protein 1 (HMW1) (MG312) - BATCH:81172

Features and Secondary Structure

1 . 10 . 20 . 30 . 40 . 50 . 60 . 70 . 80 . 90 . 100 . 110 . 120 . 130 . 140 . 150 . 160 . 170 . 180 . 190 . 200 . 210 . 220 . 230 . 240 . 250 . 260 . 270 . 280 . 290 . 300 . 310 . 320 . 330 . 340 . 350 . 360 . 370 . 380 . 390 . 400 . 410 . 420 . 430 . 440 . 450 . 460 . 470 . 480 . 490 . 500 . 510 . 520 . 530 . 540 . 550 . 560 . 570 . 580 . 590 . 600 . 610 . 620 . 630 . 640 . 650 . 660 . 670 . 680 . 690 . 700 . 710 . 720 . 730 . 740 . 750 . 760 . 770 . 780 . 790 . 800 . 810 . 820 . 830 . 840 . 850 . 860 . 870 . 880 . 890 . 900 . 910 . 920 . 930 . 940 . 950 . 960 . 970 . 980 . 990 . 1000 . 1010 . 1020 . 1030 . 1040 . 1050 . 1060 . 1070 . 1080 . 1090 . 1100 . 1110 . 1120 . 1130 .

MAKNKQSVFEEKNYTQTEPENIFGDLYDGKSTVEEDPNIKVAYDADGNGYYIAFNKETGVYYDPYGDTEYDISQLFDENGNPFVFDEKQEENDYLKYVGNPDYGSYDENGEWVWSGYFENDQWISTKESQPTDENYGFDSDLPPEVKQPESVEDNYGFDNDLPPEVKQPESVEDNYGFDNDLPPEVKQPESVVDQPSSDDYFAKQPTDENYGFDNDLPPEVKQPESVVDQPSSDDHFAKQPESTTDSYSFDSDLPQPTLDQPSLDDHVQYNFDHHEELKPVAEEQNNYQVGFDQVQANLDNNEEIQPTAEKKVTTDFESKQAQVVDSYQLPIDTDQQDQTTFSSSFETQPTVEQFDQVNSEVNDQFKPEITKEPVLESSFNKQDVVETSDLNSESNLYSENNKDATNNDSLNSEFIQLNSNSETASDDVHYESKSEPIHDYKFGSDLSQSNSNNSLESEPVKFNSETAPDAHFESQSEPVDQVQYDIYQNEELKPTLDQPSSDDYFAKQPTDENYGFDNDLPPEVKQPESVVDQPSSDDHFAKQPESTTDSYSFDSDLPQPTLDQPSLDDHVQYNFDHHEELKPVAEEQNNYQVGFDQVQANLDNNEEIQPTAEKEVTTDFESKQAQVVDSYQLPIDTDQQDQTTFSSSFETQPTVEQFDQVNSEVNDQFKPEITKEPVLESSFNKQDVVETSNYTNNLQKFDIQSDNKITITTKKSSPQIPTTLPISFVSNRIEYKPVETLALDNKESQQEQITINSITEDSKTLAKTLSVQLQQINSLNNQSIVTSESVRLDKKDDQLTINTVNSEDQQPKIEVFVKAKEPVEEHSITQNKQSVEDKSELDNFNKKSDLYKIISELKRGELNPTINFDAIFQMNDYQMSVKQSFIHLNDFVTNYKNQISERYLIIKKELQSELSRLIDQNENLNVQFNNAKNLTTLQKEEMIRSLASDFAIAYKPSNSYEQLQKSGEIMRHVQRAITENEKKIESIQGSLKQLKTVYNSCCETIMNNINKLDNTLRFAKKEKDPLLLSNFDSVTDNGLVEPNQLMDDLIDFSNTFDNISNEQLDDFIYENMDRNIDFEFEGFNNDFVDIDAKVMDSMSAFSVNDLDIETLVPDRTSNFSSLLDEDLFESSGDFSLDY

tmhmm (0)
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

low complexity (5%)
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------XXXXXXXXXXXXXX------------------------------------------XXXXXXXXXXXXXXX----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------XXXXXXXXXXXXXXXXX---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------XXXXXXXXXXXXX-------

coiled-coils (4%)
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------XXXXXXXXXXXXXXXXXXXXXXXXXXXX-------------------------------------------------------------------XXXXXXXXXXXXXXXXXXXXX--------------------------------------------------------------------------------------------------------------------

disordered (56%)
XXXXXX-XXXXX-----------------------------------------------------------------------X-XX----------------------------------------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXX----------XX-------X---XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX-------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX--------XXXXXXXXXXXXXXXXXX-----XXXXXXXXXXXXXXXXXX----------------------XXXXXXXXXX-----------------XXXXXXXXXXXXXXXXXXXXX------------------------------XXXXXXXXXXXXXXXX--------------------------------------------------------------------------------------------------------------------XXXXXXXXXXX-----------X--------------------------------------------------------------------------------------------------------------------------------------------------------X--X-X

psipred
----HHHHH---------HHHHHHHH------------EEEEE------EEEEE-----EEEE--------HHHHH-----------------HHHH-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HHH---------HH-----------------------------------------------------------------------------------------------------------------------------------------------HH---HHHH-------------------HHHHHHHHHHH---------------------------------EEE----------------------HHHHHH------HHHHHHHHH---------HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH-EEEEEE----HHHHH-----HHHHHH------HHHHHHHHHHHHH---EEE-----HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH-----HHH-------------HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH------------------------------------EE--------HHHHHHHHHHH---------

# SignalP-4.0 gram+ predictions
# Measure  Position  Value  Cutoff  signal peptide?
  max. C    36	    0.111
  max. Y     3	    0.140
  max. S     5	    0.206
  mean S     1-2    0.179
       D     1-2    0.156    0.450  NO
Name=tmp_signalp_seq	SP='NO' D=0.156 D-cutoff=0.450 Networks=SignalP-TM

Domain Repeats Prediction

Boundary STD (+/-) Consensus

-- -- --

Ginzu Domain Prediction 1

Domain Span Source Reference Parent Parent Span Confidence Annotations

1-78 alignment 1cf5A_108 1-249 0.139900 RIBOSOME-INACTIVATING PROTEIN

79-104 alignment 4dczA_301 1-55 0.685200 UNKNOWN FUNCTION

105-126 alignment 4dczA_201 1-55 0.764900 UNKNOWN FUNCTION

127-271 alignment 5kenA_201 1-207 0.0411 --

272-496 alignment 2mcqA_201 1-77 0.0328 --

497-595 alignment 1q4kA_201 1-222 0.0532 TRANSFERASE

596-737 alignment 5izhA_201 1-171 0.0524 --

738-865 alignment 4tr8B_203 1-361 0.074600 --

866-1023 alignment 5xeiA_301 1-517 0.116000 --

1024-1139 alignment 4rc1A_201 1-241 0.083300 --

Ginzu Domain Prediction 2

Domain Span Source Reference Parent Parent Span Confidence Annotations

1-78 msa 1cf5A_108 1-249 0.139900 --

79-104 alignment 4dczA_301 1-55 0.685200 UNKNOWN FUNCTION

105-126 alignment 4dczA_201 1-55 0.764900 UNKNOWN FUNCTION

127-271 cutpref -- -- 0.000000 --

272-496 msa -- -- 2.566997 --

497-595 cutpref -- -- 0.000000 --

596-737 msa -- -- 1.554994 --

738-865 msa 4tr8B_203 1-361 0.074600 --

866-1023 msa 5xeiA_301 1-517 0.116000 --

1024-1139 msa 4rc1A_201 1-241 0.083300 --

PSI-BLAST Sequence Hits (Top 20 by Identity) ALL DATA

TaxID Species Alignments PSI-Blast Data (alignment with lowest E value)

E value Query Span Sbjct Span Bit Score Identity HSP Length Accession Description

662945 Mycoplasma genitalium M6320 1 (0.16%) 0.0 1-1139 1-1139 713 100 1139 ref|NP_072979.1| HMW1 cytadherence accessory protein [Mycoplasma genitalium G...

662946 Mycoplasma genitalium M6282 1 (0.16%) 0.0 1-1139 1-1139 712 100 1139 ref|YP_006600343.1| HMW1 cytadherence accessory protein [Mycoplasma genitalium M...

2097 Mycoplasma genitalium 2 (0.31%) 2E-93 659-1139 1-481 351 100 481 ref|WP_009885890.1| HMW1 cytadherence accessory protein, partial [Mycoplasma gen...

1112856 Mycoplasma pneumoniae 309 1 (0.16%) 2E-68 285-1139 137-1018 268 25 883 ref|YP_005175689.1| cytadherence accessory protein HMW1 [Mycoplasma pneumoniae 3...

722438 Mycoplasma pneumoniae FH 1 (0.16%) 2E-68 285-1139 137-1018 268 25 883 ref|YP_005881453.1| cytadherence high molecular weight protein 1 [Mycoplasma pne...

1238993 Mycoplasma pneumoniae M129-B7 1 (0.16%) 2E-68 261-1139 152-1018 268 24 882 ref|NP_110135.1| cytadherence accessory protein HMW1 [Mycoplasma pneumoniae M...

412133 Trichomonas vaginalis G3 19 (2.98%) 2E-19 621-1063 130-582 105 20 464 ref|XP_001301913.1| hypothetical protein [Trichomonas vaginalis G3] gb|EAX88983....

7462 Apis dorsata 1 (0.16%) 0.0 4-1009 3695-4786 659 19 1139 ref|XP_006616365.1| PREDICTED: uncharacterized protein LOC102671488 [Apis dorsat...

203119 Clostridium thermocellum ATCC 27405 1 (0.16%) 3E-50 118-825 1389-2089 207 16 710 ref|YP_001039467.1| cellulosome anchoring protein cohesin subunit [Clostridium t...

492476 Clostridium thermocellum JW20 1 (0.16%) 7E-39 118-732 979-1568 170 16 615 ref|WP_003511551.1| cellulosome anchoring protein cohesin region [Clostridium th...

1515 Clostridium thermocellum 1 (0.16%) 3E-46 118-784 783-1444 194 15 669 emb|CAA47841.1| S-layer protein [Clostridium thermocellum]

637887 Clostridium thermocellum DSM 1313 1 (0.16%) 7E-42 143-786 766-1395 180 15 646 ref|YP_005687159.1| cellulosome anchoring protein cohesin subunit [Clostridium t...

1159201 Mycoplasma gallisepticum WI01_2001.043-13-2P 1 (0.16%) 1E-31 89-1123 897-1880 146 14 1051 ref|YP_006581441.1| cytadherence-associated protein [Mycoplasma gallisepticum VA...

1159200 Mycoplasma gallisepticum NY01_2001.047-5-1P 1 (0.16%) 1E-31 89-1123 897-1880 146 14 1051 ref|YP_006583726.1| cytadherence-associated protein [Mycoplasma gallisepticum NY...

1159204 Mycoplasma gallisepticum NC08_2008.031-4-3P 1 (0.16%) 2E-31 89-1123 894-1877 145 14 1051 ref|YP_006586728.1| cytadherence-associated protein [Mycoplasma gallisepticum NC...

7460 Apis mellifera 1 (0.16%) 1E-103 151-1009 3951-4851 383 13 924 ref|XP_006561030.1| PREDICTED: LOW QUALITY PROTEIN: uncharacterized protein LOC5...

10090 Mus musculus 19 (2.98%) 1E-42 228-851 546-1161 182 13 632 ref|NP_035871.2| zonadhesin precursor [Mus musculus]

1159203 Mycoplasma gallisepticum CA06_2006.052-5-2P 1 (0.16%) 7E-32 89-1123 897-1880 146 13 1051 ref|YP_006585978.1| cytadherence-associated protein [Mycoplasma gallisepticum CA...

1159202 Mycoplasma gallisepticum NC06_2006.080-5-2P 1 (0.16%) 2E-31 89-1123 897-1880 145 13 1051 ref|YP_006585225.1| cytadherence-associated protein [Mycoplasma gallisepticum NC...

708616 Mycoplasma gallisepticum str. F 1 (0.16%) 1E-26 89-1123 972-1941 129 13 1049 ref|YP_005880532.1| putative cytadherence-associated protein [Mycoplasma gallise...