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Features and Secondary Structure
	1 . 10 . 20 . 30 . 40 . 50 . 60 . 70 . 80 . 90 . 100 . 110 . 120 . 130 . 140 . 150 . 160 . 170 . 180 . 190 . 200 . 210 . 220 . 230 . 240 . 250 . 260 . 270 . 280 . 290 . 300 . 310 . 320 . 330 . 340 . 350 . 360 . 370 . 380 . 390 . 400 . 410 . 420 . 430 . 440 . 450 . 460 . 470 . 480 . 490 . 500 . 510 . 520 . 530 . 540 . 550 . 560 . 570 . 580 . 590 . 600 . 610 . 620 . 630 . 640 . 650 .
	MMDVKLKIQQLVNLIKNYDYHYYVLSEPLIDDFEYDMLYKSLQQLEKDHPDLIQIDSPTQRVGGEAVKGFKKLNHNSPMLSLENAFSTKEIANFIDNINFQTNSKNEFVVEPKIDGVSISLTYKNGVLVHALTRGDGSVGEDVLNNVKTIKSIPLTIPFTKTIEIRGEIFVDKKTFLAINNQLEKPFANARNLAAGTIRNLNSEITAQRKLRALFYYIPNGLEESITTQTMVLEQLKQWKFPVSDTIRVFQNKFQLINYLEAFDKKREQLTFNLDGLVIKLNSLLFYQQLGATSKSPRWAIAFKFSPKFVQTKLTAVLITIGRTGRVNYTAKLESVNLDGTKVTAATLHNFDYIKTKDIRINDTVVIYKAGEIIPKVLKVNLEKRKNDTIIIQEQKYCPSCNSKLVKIVDEVDQYCTNETCKERNIQLINYFVSKTAMDINGLNINTITKLYEHNLVRSIVDLYDLKDKKNQVLKLDLKIGDKLFNKLVDNIENSKQKGMARLLTGLGIKHVGNVLAKNLANHFKNIKALQHASLENLISLNDVGITVAESLYNWFHDPNHLQLIEQLELRQVKTDQLPLKINFETNSIYFQKRFLITGSFNISRDQIKDLLSAKFDCQFASEVKPTVDFVIAGNKPTLRKINHAKELNIPIINEAIWT
tmhmm (0)	-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
low complexity (0%)	-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
coiled-coils (0%)	-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
disordered (2%)	XXXX---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------XXXXXXXXXXXX----------------------------------------------------------------------
psipred	---HHHHHHHHHHHHHHHHHHHH--------HHHHHHHHHHHHHHHHH---------------------------------------HHHHHHHHHHHHHH------EEEEE----EEEEEEEE----EEEE--------EEHHHHHHHH----HH------EEEEEEEEEEHHHHHHHHHHH------HHHHH--EE------HHHH--EEEEEEEE----------HHHHHHHHHH--------EEE---HHHHHHHHHHHHHH----------EEEE---HHHHHH---------EEEEE------EEEEEEEEEEE-------EEEEEE--EEE--EEEEEEE---HHHHHH-------EEEEEE------EEEEE-----------------------EEEEE---EEEEE-----HHHHHHHHHEE------------HHHHHHHHHHH----HHHHHHHHHHHHHHHHH----HHHHHHHHHHHHHHH---HHHHHHHH-------HHHHHHHHHH---HHHHHH--HHHHHH-----HHHHHHHHHHH-----------------------------------EEEEEE-------HHHHHHHHHHH---EE-------EEEEEE-----HHHHHHHHH---EEE-HHH--

# SignalP-4.0 gram- predictions
# Measure  Position  Value  Cutoff  signal peptide?
  max. C    22	    0.113
  max. Y     2	    0.111
  max. S     1	    0.126
  mean S     1-1    0.126
       D     1-1    0.118    0.570  NO
Name=tmp_signalp_seq	SP='NO' D=0.118 D-cutoff=0.570 Networks=SignalP-noTM

Domain Repeats Prediction
Boundary	STD (+/-)	Consensus
--	--	--

Ginzu Domain Prediction 1
Domain	Span	Source	Reference Parent	Parent Span	Confidence	Annotations
	1-579	alignment	2owoA_201	1-586	0.381600	LIGASE/DNA
	580-659	alignment	2k6gA_201	1-109	0.900200	DNA BINDING PROTEIN

PSI-BLAST Sequence Hits (Top 20 by Identity)   ALL DATA
TaxID	Species	Alignments	PSI-Blast Data (alignment with lowest E value)
			E value	Query Span	Sbjct Span	Bit Score	Identity	HSP Length	Accession	Description
662947	Mycoplasma genitalium M2288	1 (0.03%)	0.0	2-659	1-658	778	100	658	ref|YP_006601285.1|	NAD-dependent DNA ligase [Mycoplasma genitalium M2288] ref|W...
722438	Mycoplasma pneumoniae FH	1 (0.03%)	0.0	2-659	1-658	785	66	658	ref|YP_005881375.1|	DNA ligase (NAD(+)) [Mycoplasma pneumoniae FH] ref|WP_014574...
340047	Mycoplasma capricolum subsp. capricolum ATCC 27343	1 (0.03%)	9E-9	132-173	1-42	69	57	42	emb|CAA83831.1|	DNA ligase [Mycoplasma capricolum subsp. capricolum ATCC 273...
431943	Clostridium kluyveri DSM 555	1 (0.03%)	9E-11	2-66	1-60	76	45	65	ref|YP_001396845.1|	DNA ligase [Clostridium kluyveri DSM 555] ref|WP_012103805.1...
73239	Plasmodium yoelii yoelii	2 (0.06%)	1E-61	3-167	186-354	245	43	169	ref|XP_728678.1|	NAD-dependent DNA ligase [Plasmodium yoelii yoelii 17XNL] gb...
550748	Ureaplasma urealyticum serovar 4 str. ATCC 27816	1 (0.03%)	0.0	1-658	1-666	775	42	668	ref|WP_004026263.1|	DNA ligase [Ureaplasma urealyticum] gb|EDX53354.1| DNA ligas...
550773	Ureaplasma urealyticum serovar 9 str. ATCC 33175	1 (0.03%)	0.0	1-658	1-666	774	42	668	ref|WP_004026844.1|	DNA ligase [Ureaplasma urealyticum] gb|EDX53870.1| DNA ligas...
515611	Ureaplasma urealyticum serovar 13 str. ATCC 33698	1 (0.03%)	0.0	1-658	1-666	770	42	668	ref|WP_004027093.1|	DNA ligase [Ureaplasma urealyticum] gb|EDT49514.1| DNA ligas...
626096	Ureaplasma urealyticum serovar 2 str. ATCC 27814	1 (0.03%)	0.0	1-658	1-666	767	42	668	ref|WP_004027361.1|	DNA ligase [Ureaplasma urealyticum] gb|EEH02061.1| DNA ligas...
626095	Ureaplasma urealyticum serovar 8 str. ATCC 27618	1 (0.03%)	0.0	1-658	1-666	767	42	668	ref|YP_002284543.1|	DNA ligase, NAD-dependent [Ureaplasma urealyticum serovar 10...
519851	Ureaplasma parvum serovar 6 str. ATCC 27818	1 (0.03%)	0.0	1-658	1-666	768	41	668	ref|WP_006688939.1|	DNA ligase [Ureaplasma parvum] gb|EDU19043.1| DNA ligase, NA...
515608	Ureaplasma parvum serovar 1 str. ATCC 27813	1 (0.03%)	0.0	1-658	1-666	767	41	668	ref|WP_006689208.1|	DNA ligase [Ureaplasma parvum] gb|EDT48865.1| DNA ligase, NA...
9606	Homo sapiens	3 (0.09%)	1E-170	4-432	16-449	605	41	434	emb|CAA66920.1|	ligase-like protein [Homo sapiens]
295996	Neisseria meningitidis alpha275	1 (0.03%)	1E-152	4-381	26-422	544	41	397	emb|CBA09270.1|	DNA ligase [Neisseria meningitidis alpha275]
391606	Carboxydibrachium pacificum DSM 12653	1 (0.03%)	3E-98	2-252	18-271	366	41	255	ref|WP_009610773.1|	NAD-dependent DNA ligase adenylation domain subfamily, parti...
1131731	Bacillus azotoformans LMG 9581	1 (0.03%)	0.0	1-656	3-660	929	40	663	ref|WP_003331579.1|	NAD-dependent DNA ligase LigA [Bacillus azotoformans] gb|EKN...
880073	Caldithrix abyssi DSM 13497	1 (0.03%)	0.0	2-658	1-662	906	40	668	ref|WP_006930949.1|	NAD-dependent DNA ligase LigA [Caldithrix abyssi] gb|EHO4334...
635013	Thermincola potens JR	1 (0.03%)	0.0	4-658	6-660	893	40	663	ref|YP_003639618.1|	NAD-dependent DNA ligase [Thermincola potens JR] ref|WP_0131...
638301	Granulicatella adiacens ATCC 49175	1 (0.03%)	0.0	2-658	5-663	891	40	664	ref|WP_005607731.1|	DNA ligase LigA [Granulicatella adiacens] gb|EEW37137.1| DNA...
945713	Ignavibacterium album JCM 16511	1 (0.03%)	0.0	2-658	3-667	857	40	671	ref|YP_005845353.1|	NAD-dependent DNA ligase [Ignavibacterium album JCM 16511] r...