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Fragment Libraries			Alanine Scanning			DNA Interface Scan
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Job 81222 [SSGCID - MygeA.20086.a] [2018-02-01] [D-172-16-30-137.dhcp4.x.x] [Structure] [Complete] Cytadherence high molecular weight protein 2 (HMW2) (MG218) - BATCH:81221

Features and Secondary Structure

1 . 10 . 20 . 30 . 40 . 50 . 60 . 70 . 80 . 90 . 100 . 110 . 120 . 130 . 140 . 150 . 160 . 170 . 180 . 190 . 200 . 210 . 220 . 230 . 240 . 250 . 260 . 270 . 280 . 290 . 300 . 310 . 320 . 330 . 340 . 350 . 360 . 370 . 380 . 390 . 400 . 410 . 420 . 430 . 440 . 450 . 460 . 470 . 480 . 490 . 500 . 510 . 520 . 530 . 540 . 550 . 560 . 570 . 580 . 590 . 600 . 610 . 620 . 630 . 640 . 650 . 660 . 670 . 680 . 690 . 700 . 710 . 720 . 730 . 740 . 750 . 760 . 770 . 780 . 790 . 800 . 810 . 820 . 830 . 840 . 850 . 860 . 870 . 880 . 890 . 900 . 910 . 920 . 930 . 940 . 950 . 960 . 970 . 980 . 990 . 1000 . 1010 . 1020 . 1030 . 1040 . 1050 . 1060 . 1070 . 1080 . 1090 . 1100 . 1110 . 1120 . 1130 . 1140 . 1150 . 1160 . 1170 . 1180 . 1190 . 1200 . 1210 . 1220 . 1230 . 1240 . 1250 . 1260 . 1270 . 1280 . 1290 . 1300 . 1310 . 1320 . 1330 . 1340 . 1350 . 1360 . 1370 . 1380 . 1390 . 1400 . 1410 . 1420 . 1430 . 1440 . 1450 . 1460 . 1470 . 1480 . 1490 . 1500 . 1510 . 1520 . 1530 . 1540 . 1550 . 1560 . 1570 . 1580 . 1590 . 1600 . 1610 . 1620 . 1630 . 1640 . 1650 . 1660 . 1670 . 1680 . 1690 . 1700 . 1710 . 1720 . 1730 . 1740 . 1750 . 1760 . 1770 . 1780 . 1790 . 1800

MKPFDKKPSLQPIYDIGFDDGYLQSEYEKNRSKTDVDKIENQLLKEIKSLEDELKNLKGLKNQAEDNPELDKKINHLEVDLNRLVNEYKNFQFQKNHMVDKVSELDNLTRFYKNELTRLQQENADFLNSKYANLANFQANYHNKLNDFHRLIENQNQTINRLNQKINGNQNLIDNNVALLQNPNITVEKKNYLLNVIDQLYNELDQLENQKRLLSIEYENTYRELVSADNELQNVYENIDQNQIQFKHQYQTYRDELSQLERKIQLTKQELVDKESALRVKIDDADFYINARLAELDDVAKQLSFQDGITKQNAQHVEDKLVALNKEKDRLNTQKEAFFNLRQSALIDINKLQQENELFAKHLEHQQNEFEQKQSDSLLKLETEYKALQHKINEFKNESATKSEELLNQERELFEKRREIDTLLTQASLEYEHQRESSQLLKDKQNEVKQHFQNLEYAKKELDKERNLLDQQKKVDSEAIFQLKEKVAQERKELEELYLVKKQKQDQKENELLFFEKQLKQHQADFENELEAKQQELFEAKHALERSFIKLEDKEKDLNTKAQQIANEFSQLKTDKSKSADFELMLQNEYENLQQEKQKLFQERTYFERNAAVLSNRLQQKREELLQQKETLDQLTKSFEQERLINQREHKELVASVEKQKEILGKKLQDFSQTSLNASKNLAEREMAIKFKEKEIEATEKQLLNDVNNAEVIQADLAQLNQSLNQERSELQNAKQRIADFHNDSLKKLNEYELSLQKRLQELQTLEANQKQHSYQNQAYFEGELDKLNREKQAFLNLRKKQTMEVDAIKQRLSDKHQALNMQQAELDRKTHELNNAFLNHDADQKSLQDQLATVKETQKLIDLERSALLEKQREFAENVAGFKRHWSNKTSQLQKIYELTKKQESEQTQKETELKIAFSDLQKDYQVFELQKDQEFRQIEAKQRELDKLAEKNNQVKLELDNRFQALQNQKQDTVQAQLELEREQHQLNLEQTAFNQANESLLKQREQLTKKIQAFHYELKKRNQFLALKGKRLFAKEQDQQRKDQEINWRFKQFEKEYTDFDEAKKRELEELEKIRRSLSQSNVELERKREKLATDFTNLNKVQHNTQINRDQLNSQIRQFLLERKNFQRFSNEANAKKAFLIKRLRSFASNLKLQKEALAIQKLEFDKRDEQQKKELQQATLQLEQFKFEKQNFDIEKQRQLVAIKTQCEKLSDEKKALNQKLVELKNLSQTYLANKNKAEYSQQQLQQKYTNLLDLKENLERTKDQLDKKHRSIFARLTKFANDLRFEKKQLLKAQRIVDDKNRLLKENERNLHFLSNETERKRAVLEDQISYFEKQRKQATDAILASHKEVKKKEGELQKLLVELETRKTKLNNDFAKFSRQREEFENQRLKLLELQKTLQTQTNSNNFKTKAIQEIENSYKRGMEELNFQKKEFDKNKSRLYEYFRKMRDEIERKESQVKLVLKETQRKANLLEAQANKLNIEKNTIDFKEKELKAFKDKVDQDIDSTNKQRKELNELLNENKLLQQSLIERERAINSKDSLLNKKIETIKRQLHDKEMRVLRLVDRMKLAEQKYQTEINRLRTQTFDSEKQDIKNFFPPLFKINGNDMAFPYLYPWLYPQQKQDDNTLQIRQLFEQQLQFMQQRYENELNELRRQRNLLEKKLDQIQLESQLNNKQSEFSKVESMMEKLLEKTESRLNDFDQKINYLTKKVNQHNTYQPSSYQPTPSYQDSDKQQLLFRIQELEKQNLFQQQFQPAPAVVQQPTSFAAPNITKQQQIAQLNAEINNIKRLIAQKAASK

tmhmm (0)
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

low complexity (7%)
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------XXXXXXXXXXXXXX----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------XXXXXXXXXXXXXXXXXX----------------------------------------------------------------------------------------------------------------------------------------------------XXXXXXXXXXXXXXXXX--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------XXXXXXXXXXXXXX---------------------------------------------------------------------------------------------------------XXXXXXXXXXXXXXXXXXX---------------------------------------------------------------------------------------------------------XXXXXXXXXXXXXXXXXXXXXXXXXXXXX----------------------------------------------------------------------------------------XXXXXXXXXXXXXX------------------------------------

coiled-coils (58%)
---------------------------------------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX-------XXXXXXXXXXXXXXXXXXXXX----------------------XXXXXXXXXXXXXXXXXXXXXXXXXXXX-----------------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX----XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX------------------------------------XXXXXXXXXXXXXXXXXXXXX--------------XXXXXXXXXXXXXXXXXXXXX--------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX--------------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX----XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX--------------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX---------------------------------------XXXXXXXXXXXXXXXXXXXXX-------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX-XXXXXXXXXXXXXXXXXXXXXXXX---------------XXXXXXXXXXXXXXXXXXXXX----XXXXXXXXXXXXXXXXXXXXX------------------------------------------------------------------------------------------------------XXXXXXXXXXXXXXXXXXXXXXXXXXXX----XXXXXXXXXXXXXXXXXXXXXXXX---------------------------------------------------------------------------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX---------------------------------------------------------------------------------------------------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX-------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX---------------------XXXXXXXXXXXXXXXXXXXXX-----------------------------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX------------------------XXXXXXXXXXXXXXXXXXXXX-----------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX-----------------------------XXXXXXXXXXXXXXXXXXXXX----------------------------------------------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX--XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX-----------------------------------------------------------XXXXXXXXXXXXXXXXXXXXXXXXXXXX-

disordered (27%)
XXXXXX-X-------------------XXXXXXXXXXX-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------XXXXXXXXXXXXXXXXXXXXX-------------XXXXXXXXXXXXXXX------------------XXXXXXXXXXXXXXXXXXXXXX--------XXXXXXXXXXXXXXX----------X--XXXXX----XXXXXXXXXXXXXXXXXX-------------------XXXXXXXXXXXXXXXXX--------------------------------------------------------------------------------------------------------------------------XXXXXXXXX---------------------------------------------------------------------------------XXXXXXXXXXXXXXX------------------------------------------------------------------------------------------------------------------------XXXXXXXXXXXXXXXXXX-----------------------------------------------------XXXXXXXXXXXX-------------------------------XXXXXXXXXXXXXXXX------XXXXXXXXXXXXXXXXXXXXX----------------XXXXXXXXXX-------------------------------------------------XXXXXXXXXXXXXXXXX---XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX----------------------------------------------------------XXXXX---------------------------------------------XXXXXXXXXXXXXXXXXXXXXX---XXXXXXXXXXXXXXXXX-----XX--XXXXX-XXXX-XX-X---------------------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX-----------XXXXXXXXXXXXXXXX------------XXXXXXX-X------------------------------XXXXXXXXXXXXXXXXXXXXXXXXXXXX------------------------------------------------------------------------------------------------------X-XXXXXXXX--X------------------------------------XXXXXXXXXXXXXXXXXXX---------------------------------------------------------XXXXXXXXXXXXXXXX--XXX---XXX--XXXX------------------XXXX

psipred
---------------------HHHHHHHHHHHH-HHHHHHHHHHHHHHHHHHHHHHHH----------HHHHHHHHHHHHHHHHHHHHH---HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH--HHHHHHHHHH------HHHHHHHH------HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH--HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH-----HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH---HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH----HHHHHHHH-HHHHH-----HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH-------HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH--HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH----HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH----HHHHHHHHHHHHHHHHHHHHHH-----HHHHHHHHHHHHHHHHHHHHH-HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH----HHHHHHHHHHHHHHHHHHHHHHH---HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH---HHHHHHHHHHHHHH------HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH--HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH-----HHHH--HHHHHH---------------HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH------HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH------------------HHHHHHHHHHHHHHHHHHHH------HHHH------------HHHHHHHHHHHHHHHHHHHHHH---

# SignalP-4.0 gram- predictions
# Measure  Position  Value  Cutoff  signal peptide?
  max. C    65	    0.108
  max. Y    43	    0.111
  max. S    33	    0.136
  mean S     1-42   0.098
       D     1-42   0.105    0.570  NO
Name=tmp_signalp_seq	SP='NO' D=0.105 D-cutoff=0.570 Networks=SignalP-noTM

Domain Repeats Prediction

Boundary STD (+/-) Consensus

-- -- --

Ginzu Domain Prediction 1

Domain Span Source Reference Parent Parent Span Confidence Annotations

1-75 alignment 1l8dA_304 1-103 0.450000 REPLICATION

76-345 alignment 2tmaA_101 1-284 0.099100 CONTRACTILE SYSTEM PROTEIN

346-412 alignment 1ei3B_309 1-395 0.3459 BLOOD CLOTTING

413-1805 alignment 3vkgA_201 1-2954 0.049200 motor protein

Ginzu Domain Prediction 2

Domain Span Source Reference Parent Parent Span Confidence Annotations

1-75 alignment 1l8dA_304 1-103 0.450000 REPLICATION

76-332 cutpref -- -- 0.000000 --

333-645 msa -- -- 1.490984 --

646-705 cutpref -- -- 0.000000 --

706-840 cutpref -- -- 0.000000 --

841-1085 cutpref -- -- 0.000000 --

1086-1194 cutpref -- -- 0.000000 --

1195-1248 alignment 2tmaA_301 1-284 0.885200 CONTRACTILE SYSTEM PROTEIN

1249-1495 msa -- -- 1.500996 --

1496-1737 cutpref -- -- 0.000000 --

1738-1805 alignment 1l8dA_309 1-103 0.333300 REPLICATION

PSI-BLAST Sequence Hits (Top 20 by Identity) ALL DATA

TaxID Species Alignments PSI-Blast Data (alignment with lowest E value)

E value Query Span Sbjct Span Bit Score Identity HSP Length Accession Description

662946 Mycoplasma genitalium M6282 1 (0.06%) 0.0 1-1805 1-1805 675 100 1805 ref|YP_006600246.1| HMW2 cytadherence accessory protein [Mycoplasma genitalium M...

662945 Mycoplasma genitalium M6320 1 (0.06%) 0.0 1-1805 1-1805 675 100 1805 ref|NP_072883.1| HMW2 cytadherence accessory protein [Mycoplasma genitalium G...

662947 Mycoplasma genitalium M2288 1 (0.06%) 0.0 1-1805 1-1805 674 100 1805 ref|YP_006601248.1| HMW2 cytadherence accessory protein [Mycoplasma genitalium M...

1112856 Mycoplasma pneumoniae 309 1 (0.06%) 1E-147 1-1803 1-1817 532 56 1818 ref|YP_005175549.1| cytadherence accessory protein HMW2 [Mycoplasma pneumoniae 3...

2104 Mycoplasma pneumoniae 2 (0.12%) 1E-147 1-1803 1-1817 531 56 1818 ref|WP_019830459.1| Cytadherence high molecular weight protein 2 [Mycoplasma pne...

1238993 Mycoplasma pneumoniae M129-B7 1 (0.06%) 1E-147 1-1803 1-1817 530 56 1818 ref|NP_109998.1| cytadherence protein [Mycoplasma pneumoniae M129] ref|YP_007...

9925 Capra hircus 5 (0.31%) 1E-117 332-1434 845-1931 431 17 1127 ref|XP_005693631.1| PREDICTED: myosin-8 [Capra hircus]

708616 Mycoplasma gallisepticum str. F 1 (0.06%) 1E-83 20-1779 74-1852 319 17 1791 ref|YP_005880786.1| cytadherence-associated protein Hlp2 [Mycoplasma galliseptic...

9823 Sus scrofa 10 (0.62%) 1E-119 332-1434 844-1930 437 16 1127 ref|NP_001116613.1| myosin-4 [Sus scrofa] sp|Q9TV62.1|MYH4_PIG RecName: Full=Myo...

10141 Cavia porcellus 10 (0.62%) 1E-119 332-1434 846-1932 437 16 1127 ref|XP_005002149.1| PREDICTED: LOW QUALITY PROTEIN: myosin-4 [Cavia porcellus]

9483 Callithrix jacchus 13 (0.8%) 1E-119 332-1437 847-1936 437 16 1130 ref|XP_002747955.2| PREDICTED: myosin-1 [Callithrix jacchus]

43179 Ictidomys tridecemlineatus 6 (0.37%) 1E-119 332-1437 849-1938 436 16 1130 ref|XP_005332935.1| PREDICTED: myosin-1 [Ictidomys tridecemlineatus]

9615 Canis lupus familiaris 17 (1.05%) 1E-119 332-1437 846-1935 436 16 1130 ref|XP_005619524.1| PREDICTED: myosin-1 isoform X1 [Canis lupus familiaris] sp|Q...

89462 Bubalus bubalis 7 (0.43%) 1E-119 332-1437 845-1934 436 16 1130 ref|XP_006055109.1| PREDICTED: myosin-2 [Bubalus bubalis]

30538 Vicugna pacos 6 (0.37%) 1E-118 332-1437 849-1938 436 16 1130 ref|XP_006216240.1| PREDICTED: myosin-4 [Vicugna pacos]

10090 Mus musculus 82 (5.07%) 1E-118 332-1434 846-1932 436 16 1127 ref|NP_034985.2| myosin-4 [Mus musculus] sp|Q5SX39.1|MYH4_MOUSE RecName: Full...

9685 Felis catus 6 (0.37%) 1E-119 332-1437 845-1934 436 16 1130 ref|XP_003996262.1| PREDICTED: myosin-2 isoform X1 [Felis catus] ref|XP_00693985...

79684 Microtus ochrogaster 9 (0.56%) 1E-119 332-1434 847-1933 436 16 1127 ref|XP_005349900.1| PREDICTED: myosin-4 [Microtus ochrogaster]

9978 Ochotona princeps 6 (0.37%) 1E-118 332-1434 845-1931 436 16 1127 ref|XP_004594798.1| PREDICTED: myosin-4 isoform X3 [Ochotona princeps]

230844 Peromyscus maniculatus bairdii 8 (0.5%) 1E-118 332-1434 847-1933 436 16 1127 ref|XP_006973574.1| PREDICTED: myosin-4 isoform X1 [Peromyscus maniculatus baird...