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Fragment Libraries			Alanine Scanning			DNA Interface Scan
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Job 82015 [SSGCID - ChtrB.17928.a] [2018-03-09] [D-172-16-30-137.dhcp4.x.x] [Structure] [Complete] Glycine--tRNA ligase (Glycyl-tRNA synthetase) (GlyRS) (EC 6.1.1.14) [Includes: Glycine--tRNA ligase alpha subunit (Glycyl-tRNA synthetase alpha subunit) Glycine--tRNA ligase beta subunit (Glycyl-tRNA synthetase beta subunit)] - BATCH:82003

Features and Secondary Structure

1 . 10 . 20 . 30 . 40 . 50 . 60 . 70 . 80 . 90 . 100 . 110 . 120 . 130 . 140 . 150 . 160 . 170 . 180 . 190 . 200 . 210 . 220 . 230 . 240 . 250 . 260 . 270 . 280 . 290 . 300 . 310 . 320 . 330 . 340 . 350 . 360 . 370 . 380 . 390 . 400 . 410 . 420 . 430 . 440 . 450 . 460 . 470 . 480 . 490 . 500 . 510 . 520 . 530 . 540 . 550 . 560 . 570 . 580 . 590 . 600 . 610 . 620 . 630 . 640 . 650 . 660 . 670 . 680 . 690 . 700 . 710 . 720 . 730 . 740 . 750 . 760 . 770 . 780 . 790 . 800 . 810 . 820 . 830 . 840 . 850 . 860 . 870 . 880 . 890 . 900 . 910 . 920 . 930 . 940 . 950 . 960 . 970 . 980 . 990 . 1000

MSSQPLTLQTMMAAILNFWSEQGCIIHQGYDLEVGAGTFNPATFLQSLGPEPFRTAYIEPSRRPQDGRYGQHPNRLQKYHQLQVILKPVPENFLSLYLESLKVIGLNLVDHDIRFVHDDWENPTIGAWGLGWEVWLNGMEITQLTYFQAVGSKPLDAISGEITYGVERIAMYLQKKNSVYDVMWNDSLTYGDITQYAEQAWSQYNFETANTTMWLKHFDDFSAEALATLDQGLPLPAYDFVIKASHAFNMLDSRGVISVTERTRYIAKIRQLARAAADKYVAWRESLGFPLLKTPPSTPTVTPKKIPTICQPEDFLLEIGSEELPATFVPTGIQQLESLAKKLLADHGIAYKHLEVLGTPRRLALCIEGLSHVTIRPESEKKGPPLSLLFMTDGSVSPQGEQFFSSHGLSISHRSALDQPSAIWRVRSINGTDYLFLVIPEERKETAAILVNELPQLIRSIRFPQKMTWDNGGVEYARPIRWLVALYGDQILPISLGFVSSGNTSWGHRQLDNRQLTIPSSNMYVDTLRSACVIVSQKERRAIIKQGLQNLTGDQIVAIAPEHLIDETVFLTEHPFVISAQFDPAFCSLPKELLIAEMIQHQRYFPTQNMQGEITNRFLIVCDNSPTDSIVEGNEKALAPRLTDGNFLFKQDLLTPLSSFVEKLKSVTYFESLGSLADKTSRLKLHLEEAYALLPLCAKEDIDTAIHYCKADLVSSVVNEFPELQGIMGRYYLQNASLSRAAALAIGEHLQHITLGSSISTTGALLSILDRIDNLLSCFILGLLPTSSHDPYALRRQSLEILTLLYTTQSSVDIEDLFARLIRHFPSSIPNTVWSPEAVLSKLNTFVWGRLRTILSSLGFDKEVIATVLTDNCPKNPLTIIQSAQSIQELKNTQILKTIAAMYNRLKKILASLSFSVTEQMFSLQSAEDLLFKQALDRFVEETTALPISSKDYLHLLKELAQSTELFLDSVRVASDDESTRNQRIALLIAAQKCFGFYAWGVL

tmhmm (0)
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

low complexity (2%)
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------XXXXXXXXXXXXXXXX-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

coiled-coils (0%)
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

disordered (4%)
XXXXX--XX-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------XXXXXXXXXXXXXXXXXXXXXXXX-------------------------------------------------------------XXXXXX----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

psipred
-------HHHHHHHHHHHHHH---EEE-------------HHHHHHH-----EEEEEE---------------HHHHHHHHEEEEE----HHHHHHHHHHHHH----------EEE---------------EEEEE--EEEEHHHHHHHH---------------HHHHHHHHH---EEEEEE-----EEHHHHHHHHHHHHHHHHHH--HHHHHHHHHHHHHHHHHHHH-----HHHHHHHHHHHHHHHHHH-----HHHHHHHHHHHHHHHHHHHHHHHHHHHH-------------------------HHHHHHHHHH----HHHHHHHHHHHHHHHHHHHHH--------EEEE---EEEEEEEE---------EEE---HHHHH-------HHHHHHHHHH----HHHHHHHH-----EEEEE-----EEEEEEEE----HHHHHHHHHHHHHH------EEEE----EEEE-HHHHHHHH--------EE--EEE--EE----------EEE--HHHHHHHHH---EE--HHHHHHHHHHHHHHHHHH-------HHHHHHHH------EEEEEEE-HHH----HHHHHHHHHHHEEEEEEE---------EEEE------HHHHHHHHHHHHHHHHHHHHHHHH-----HHHHHHHHH----HHH---HHHHHHHHHHHHHHHHHHH---HHHHHHHHHHHHHHHHHHHHHH--HHHHHHHHHHHHHH----HHHHHHHHHHH-----------HHHHHHHHHHHHHHHHHHH----------HHHHHHHHHHHHHHHHH------HHHHHHHHHHHHHHHH------HHHHHHHHHHHHHHHHHHHHHH----HHHHHHHH-------HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH-------HHHHH--HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH--EEEE---HHHHHHHHHHHHHHHHHHHHHHHHH-

# SignalP-4.0 euk predictions
# Measure  Position  Value  Cutoff  signal peptide?
  max. C    24	    0.130
  max. Y    24	    0.214
  max. S     5	    0.509
  mean S     1-23   0.352
       D     1-23   0.289    0.450  NO
Name=tmp_signalp_seq	SP='NO' D=0.289 D-cutoff=0.450 Networks=SignalP-noTM

Domain Repeats Prediction

Boundary STD (+/-) Consensus

-- -- --

Ginzu Domain Prediction 1

Domain Span Source Reference Parent Parent Span Confidence Annotations

1-296 alignment 3wqyB_304 1-905 0.959300 --

297-383 alignment 4x4rA_301 1-443 0.443200 --

384-674 alignment 4v36A_308 1-326 0.022700 --

675-786 alignment 2ogiA_303 1-193 0.210000 HYDROLASE

787-1003 alignment 5b63A_301 1-563 0.157600 --

Ginzu Domain Prediction 2

Domain Span Source Reference Parent Parent Span Confidence Annotations

1-296 alignment 3wqyB_304 1-905 0.959300 --

297-442 alignment 4x4rA_301 1-443 0.443200 --

443-626 cutpref -- -- 0.000000 --

627-874 msa 2ogiA_303 1-193 0.210000 --

875-950 alignment 1k40A_306 1-126 0.3438 TRANSFERASE

951-1003 alignment 4dndA_310 1-95 0.7009 TRANSPORT PROTEIN

PSI-BLAST Sequence Hits (Top 20 by Identity) ALL DATA

TaxID Species Alignments PSI-Blast Data (alignment with lowest E value)

E value Query Span Sbjct Span Bit Score Identity HSP Length Accession Description

813 Chlamydia trachomatis 5 (0.15%) 0.0 1-1003 1-1003 1269 100 1003 ref|NP_220316.1| Glycyl tRNA Synthetase [Chlamydia trachomatis D/UW-3/CX] ref...

759364 Chlamydia trachomatis D-LC 1 (0.03%) 0.0 1-1003 29-1031 1267 100 1003 ref|YP_005811847.1| Glycyl-tRNA synthetase [Chlamydia trachomatis D-EC] ref|YP_0...

718219 Chlamydia trachomatis G/9301 1 (0.03%) 0.0 1-1003 1-1003 1267 100 1003 ref|YP_005808183.1| glycyl-tRNA synthetase [Chlamydia trachomatis G/9768] ref|YP...

707186 Chlamydia trachomatis G/11222 1 (0.03%) 0.0 1-1003 1-1003 1265 100 1003 ref|YP_005809110.1| glycyl-tRNA synthetase [Chlamydia trachomatis G/11222] ref|W...

580049 Chlamydia trachomatis B/Jali20/OT 1 (0.03%) 0.0 1-1003 1-1003 1264 100 1003 ref|YP_328625.1| glycyl-tRNA synthetase [Chlamydia trachomatis A/HAR-13] ref|...

1071753 Chlamydia trachomatis A/363 1 (0.03%) 0.0 1-1003 1-1003 1263 100 1003 ref|YP_002889302.1| glycyl-tRNA synthetase [Chlamydia trachomatis B/TZ1A828/OT] ...

1071758 Chlamydia trachomatis D/SotonD6 1 (0.03%) 0.0 1-1003 1-1003 1263 100 1003 ref|YP_007731643.1| glycyl-tRNA synthetase [Chlamydia trachomatis K/SotonK1] ref...

580047 Chlamydia trachomatis A2497 1 (0.03%) 0.0 1-1003 29-1031 1261 100 1003 ref|YP_005813689.1| Glycyl-tRNA synthetase [Chlamydia trachomatis A2497] ref|WP_...

1007870 Chlamydia trachomatis RC-L2/55 1 (0.03%) 0.0 1-1003 1-1003 1266 99 1003 ref|YP_001654256.1| glycyl-tRNA synthetase [Chlamydia trachomatis 434/Bu] ref|YP...

907269 Chlamydia trachomatis RC-F(s)/342 1 (0.03%) 0.0 1-1003 1-1003 1265 99 1003 ref|YP_008345804.1| glycyl-tRNA synthetase [Chlamydia trachomatis RC-F(s)/852] r...

1100832 Chlamydia trachomatis E/C599 1 (0.03%) 0.0 1-1003 1-1003 1265 99 1003 ref|YP_005810035.1| glycyl-tRNA synthetase [Chlamydia trachomatis E/11023] ref|Y...

907270 Chlamydia trachomatis RC-J(s)/122 1 (0.03%) 0.0 1-1003 1-1003 1264 99 1003 ref|YP_008348610.1| glycyl-tRNA synthetase [Chlamydia trachomatis RC-J(s)/122] r...

1100833 Chlamydia trachomatis F/SWFPminus 1 (0.03%) 0.0 1-1003 1-1003 1263 99 1003 ref|YP_008655967.1| glycyl-tRNA synthetase [Chlamydia trachomatis F/SWFPminus] r...

243161 Chlamydia muridarum str. Nigg 1 (0.03%) 0.0 1-1003 1-1003 1226 88 1003 ref|NP_296557.1| glycyl-tRNA synthetase, tetrameric type, alpha/beta subunits...

264202 Chlamydophila felis Fe/C-56 1 (0.03%) 0.0 3-1003 2-1005 1197 66 1006 ref|YP_515108.1| glycyl tRNA synthetase [Chlamydophila felis Fe/C-56] ref|WP_...

1003238 Chlamydophila abortus LLG 1 (0.03%) 0.0 3-1003 2-1003 1203 65 1005 ref|WP_006344398.1| glycyl-tRNA synthetease [Chlamydophila abortus] gb|EGK69526....

218497 Chlamydophila abortus S26/3 1 (0.03%) 0.0 3-1003 2-1003 1202 65 1005 ref|YP_220182.1| glycyl-tRNA synthetase alpha chain [Chlamydophila abortus S2...

1112251 Chlamydia psittaci 99DC5 1 (0.03%) 0.0 3-1003 2-1003 1198 65 1005 ref|YP_007438549.1| glycyl-tRNA synthetase alpha chain [Chlamydia psittaci Mat11...

1112248 Chlamydia psittaci C1/97 1 (0.03%) 0.0 3-1003 2-1003 1195 65 1005 ref|YP_006734550.1| glycyl-tRNA synthetase subunit alpha [Chlamydia psittaci GR9...

1112253 Chlamydia psittaci 02DC14 1 (0.03%) 0.0 3-1003 2-1003 1195 65 1005 ref|YP_004422646.1| glycyl-tRNA synthetase [Chlamydia psittaci 6BC] ref|YP_00566...