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Fragment Libraries			Alanine Scanning			DNA Interface Scan
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Job 88338 [SSGCID - MygeA.20030.a] [2019-01-18] [D-172-16-30-137.dhcp4.x.x] [Structure] [Complete] Uncharacterized ATP-dependent helicase MG018 (MG_018) - BATCH:88256

Features and Secondary Structure

1 . 10 . 20 . 30 . 40 . 50 . 60 . 70 . 80 . 90 . 100 . 110 . 120 . 130 . 140 . 150 . 160 . 170 . 180 . 190 . 200 . 210 . 220 . 230 . 240 . 250 . 260 . 270 . 280 . 290 . 300 . 310 . 320 . 330 . 340 . 350 . 360 . 370 . 380 . 390 . 400 . 410 . 420 . 430 . 440 . 450 . 460 . 470 . 480 . 490 . 500 . 510 . 520 . 530 . 540 . 550 . 560 . 570 . 580 . 590 . 600 . 610 . 620 . 630 . 640 . 650 . 660 . 670 . 680 . 690 . 700 . 710 . 720 . 730 . 740 . 750 . 760 . 770 . 780 . 790 . 800 . 810 . 820 . 830 . 840 . 850 . 860 . 870 . 880 . 890 . 900 . 910 . 920 . 930 . 940 . 950 . 960 . 970 . 980 . 990 . 1000 . 1010 . 1020 . 1030

MTVAEIKKLALNNQVFNEAKALLEKGNVIFPKKFLKRKKIIIEVLDGKVFKVQINLKTAAAHLDCSCSNDKQNCVHIIAALLKYNDLKNQDNKEFDLNKADKLECKEVEILIENVSLAIVNGSWKLKIGFVINIDKVQTNTTALRFYCCDNKDVYFLHTEDEQLFRIALDKFNSVERQTLLIFDQLNKTKQMQYENNSLLFNLDQFLSLVKEVKKPSLFLLNEDKTDNILFLRSQHKINGLSHVCGFLNNKVFDFVSYNEKTKQIVLRLAYLNKFTDFKFPYNINIYKLAFGETLFFHFLIHLKMNGFKNIFFQSDVVIVKESEYLPKMFLTIEFNTQKNKFITDAFFKYKNKNSNTLTTVYPHRYYLAQKTNTSNFNRLLFYEQALQRFYEELFQIDYLRRFENIPIKDKNQIALFKTVFDDYKTIDLAELKLTSNLLNYKQLHFSISDIKALKIEDRQLKIEFKAGGIDLKLIKSVLSNYYKGNAICIGEDGWYDLNDENAKALISFWSQIDLRNATCDANNNLLLAKYHLFEVVDTISKYTDVTNLLDEKTALQLKIASENQFHLSLDNNQINNLRKYQKEGVKWIRALEDNQFGGILADEMGLGKTAQVIFAMLDSYQSTKSLLPSLIIVPASLLLNWKSEFQKFAPHVKIVTANGNFKERSQVYESLKNQILLMSFNVLRSDIKWISQKKFHYVVIDEAQGIKNENSTVTKAAKKIKGNFCLALTGTPIENRLLDLWSCFDFVLPNFLGNKKQFSDQFEKEKNDESFQKLMKKTSPFILRRTKNKVLKELPKKIITDIYVELSEEHQKLYDKQKTDGLKEIKESDAKNALNILSLILKLRHICSLVKDNDVNDFEDNSKANAALNIIYEALENKRKVILFTQFLDVIDCFKQTLKNQKIDHLVFDGRKTVKNRNTIIQKFNSAKEPCVMLASLKAGGVGINLTAAEVVIHFDVWWNSAVENQATDRAHRIGQSKTVQVYRIIAKNTIEERVCQVQNQKQELVKKTLVEDVNFFKSLSHEELLKLFE

tmhmm (0)
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

low complexity (0%)
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

coiled-coils (0%)
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

disordered (8%)
X---------------------------------------------------------------------------------------XXXXXXXXXXXXXXXXXXXXXXXXX---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------X-XXX----------------------------------------------------------------XXXXXXXXXX--------------------XX--------------------------------------------------------------------------XXXXX------------------------------------------------------------------------------------------------------------------------------------------XXXXXXXXX-----------------------------------------------XXXXXXXXXXXXXXX---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------XXXXXXX------------

psipred
--HHHHHHH---HHHHHHHHHHHH---EEEE-------EEEEEEE--EEEEEEEE-----EEEE----------HHHHHHHHHHHHHHHHHH--HHHHHHHHHH--HHHHH-----HHHH----EE-EEEEEEE------HHHHEEEEE--HHHHHHH-----EEEHHHHH--HHHHHHHHHHHHHHHHHHH-----EEEE-HHHHHHHHHHH----EEEE-------HHHHHHHH----HHHHHHH-------HHHHHHH--EEEE-HHHHHHHHHHHHH------HHHHHHHHHHHHHHHHHH----EEEE--HHHHHHHHH----EEEEEEE-----EEEEEEEEE---------------HHHHH----HHHHHHHHHHHHHHHHHHHHHHH----HHHHH-----HHHHHHHHHHHHHHHHH---EEEE-HHHH--HH-----------------EEEEEEE----HHHHHHHHHHHH----EEEE--EEEEE-HHHHHHHHHHHHH---HH----------HHHHHHHHHHHHHHHH--HHHHHHHHHHHHH-------------HHH-----HHHHHHHHHHHHHHH-----EEE--------HHHHHHHHHHHHH------EEEEE-HHHHHHHHHHHHHH----EEEEEE--HHHHHHHHHH----EEE--HHHHHHHHHHHH-----EEEEE-HHH---HHHHHHHHHHH-----EEEEE-------HHHHHHHHHHH-------HHHHHHHH-----HHHHHHHHHHHHHHH-EEEHHHHHHH----EEEEEE----HHHHHHHHHHHHH-HHHHHH---HHHHHHHHHHHHHHHHH---------------HHHHHHHHHHHHH------EEE--HHHHHHHHHHHHHH----EEEE-----HHHHHHHHHH-------EEEEE---------------EEEEE-----HHHHHHHHHHHHHH----EEEEEEE-----HHHHHHHHHHHHHHHHHHHHH----HH----HHHHHHHH-

# SignalP-4.0 gram+ predictions
# Measure  Position  Value  Cutoff  signal peptide?
  max. C    22	    0.153
  max. Y    22	    0.174
  max. S    17	    0.288
  mean S     1-21   0.167
       D     1-21   0.171    0.450  NO
Name=tmp_signalp_seq	SP='NO' D=0.171 D-cutoff=0.450 Networks=SignalP-TM

Domain Repeats Prediction

Boundary STD (+/-) Consensus

-- -- --

Ginzu Domain Prediction 1

Domain Span Source Reference Parent Parent Span Confidence Annotations

1-93 alignment m346A_101 1-100 0.847600 --

94-261 alignment 4cknC_102 1-181 0.097700 --

262-543 alignment 4j8sA_201 1-180 0.0276 PROTEIN BINDING

544-1031 alignment 5jxrA_101 1-625 0.325300 --

Ginzu Domain Prediction 2

Domain Span Source Reference Parent Parent Span Confidence Annotations

1-93 msa m346A_101 1-100 0.847600 --

94-261 msa 4cknC_102 1-181 0.097700 --

262-543 cutpref -- -- 0.000000 --

544-1031 alignment 5jxrA_101 1-625 0.325300 --

PSI-BLAST Sequence Hits (Top 20 by Identity) ALL DATA

TaxID Species Alignments PSI-Blast Data (alignment with lowest E value)

E value Query Span Sbjct Span Bit Score Identity HSP Length Accession Description

488339 synthetic Mycoplasma genitalium JCVI-1.0 1 (0.03%) 0.0 1-1031 1-1031 793 100 1031 ref|NP_072678.2| SNF2 family helicase [Mycoplasma genitalium G37] ref|WP_0108...

663918 Mycoplasma genitalium M2321 1 (0.03%) 0.0 1-1031 1-1031 793 100 1031 ref|YP_006599535.1| SNF2 family helicase [Mycoplasma genitalium M2321] ref|WP_01...

662947 Mycoplasma genitalium M2288 1 (0.03%) 0.0 1-1031 1-1031 792 100 1031 ref|YP_006601027.1| SNF2 family helicase [Mycoplasma genitalium M2288] ref|WP_01...

662946 Mycoplasma genitalium M6282 1 (0.03%) 0.0 1-1009 1-1009 777 100 1009 ref|YP_006600031.1| SNF2 family helicase [Mycoplasma genitalium M6282] ref|WP_01...

662945 Mycoplasma genitalium M6320 1 (0.03%) 0.0 1-1009 1-1009 776 100 1009 ref|YP_006600518.1| SNF2 family helicase [Mycoplasma genitalium M6320] ref|WP_01...

1112856 Mycoplasma pneumoniae 309 1 (0.03%) 0.0 1-1031 1-1030 729 62 1032 ref|YP_005175252.1| SNF2 family helicase [Mycoplasma pneumoniae 309] ref|YP_0058...

1238993 Mycoplasma pneumoniae M129-B7 1 (0.03%) 0.0 1-1031 1-1030 728 62 1032 ref|NP_109708.1| helicase-like protein [Mycoplasma pneumoniae M129] ref|YP_00...

353496 Lactobacillus delbrueckii subsp. bulgaricus 2038 1 (0.03%) 1E-135 431-1031 34-637 489 35 613 ref|YP_005852384.1| SNF2-related domain:Helicase, C-terminal:SWIM Zn-finger [Lac...

702452 Listeria seeligeri FSL S4-171 1 (0.03%) 1E-121 525-1031 2-505 443 34 513 gb|EFS03000.1| DNA/RNA helicase protein [Listeria seeligeri FSL S4-171]

312017 Tetrahymena thermophila SB210 1 (0.03%) 1E-33 828-1029 1335-1536 152 34 202 ref|XP_977122.2| SNF2 family N-terminal domain containing protein [Tetrahymen...

629262 Pseudomonas syringae pv. japonica str. M301072 1 (0.03%) 1E-133 495-1030 6-547 484 33 550 gb|EGH29045.1| SNF2-related:helicase, partial [Pseudomonas syringae pv. jap...

329726 Acaryochloris marina MBIC11017 4 (0.1%) 1E-126 453-1031 828-1405 459 33 593 ref|YP_001515515.1| SNF2 family helicase [Acaryochloris marina MBIC11017] ref|WP...

63737 Nostoc punctiforme PCC 73102 2 (0.05%) 1E-122 444-1029 819-1399 448 33 597 ref|YP_001870198.1| SNF2-related protein [Nostoc punctiforme PCC 73102] ref|WP_0...

445973 Clostridium bartlettii DSM 16795 1 (0.03%) 1E-110 436-1031 111-697 405 33 601 ref|WP_007286902.1| hypothetical protein [[Clostridium] bartlettii] gb|EDQ96043....

1042400 Lactobacillus delbrueckii subsp. bulgaricus CNCM I-1519 1 (0.03%) 1E-136 360-1031 86-757 494 32 685 ref|WP_004560795.1| serine/threonine protein kinase [Lactobacillus delbrueckii] ...

1144300 Lactobacillus gastricus PS3 1 (0.03%) 1E-135 406-1031 1-634 490 32 638 ref|WP_007122223.1| Putative helicase [Lactobacillus gastricus] gb|EHS86500.1| P...

381754 Pseudomonas aeruginosa PA7 4 (0.1%) 1E-131 495-1030 222-766 475 32 552 ref|YP_001350061.1| helicase [Pseudomonas aeruginosa PA7] ref|WP_012077012.1| he...

350703 Pseudomonas aeruginosa 2192 2 (0.05%) 1E-130 495-1030 112-656 473 32 552 ref|WP_004353123.1| helicase [Pseudomonas aeruginosa] gb|EAZ62493.1| hypothetica...

1163396 Pseudomonas aeruginosa CI27 2 (0.05%) 1E-130 495-1030 112-656 473 32 552 ref|YP_792483.1| helicase [Pseudomonas aeruginosa UCBPP-PA14] ref|WP_00314091...

350704 Pseudomonas aeruginosa C3719 2 (0.05%) 1E-130 495-1030 112-656 473 32 552 ref|WP_004348251.1| helicase [Pseudomonas aeruginosa] gb|EAZ56599.1| hypothetica...