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Fragment Libraries			Alanine Scanning			DNA Interface Scan
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Job 88349 [SSGCID - MygeA.20070.a] [2019-01-18] [D-172-16-30-137.dhcp4.x.x] [Structure] [Complete] Uncharacterized ATP-dependent helicase MG140 (MG_140) - BATCH:88256

Features and Secondary Structure

1 . 10 . 20 . 30 . 40 . 50 . 60 . 70 . 80 . 90 . 100 . 110 . 120 . 130 . 140 . 150 . 160 . 170 . 180 . 190 . 200 . 210 . 220 . 230 . 240 . 250 . 260 . 270 . 280 . 290 . 300 . 310 . 320 . 330 . 340 . 350 . 360 . 370 . 380 . 390 . 400 . 410 . 420 . 430 . 440 . 450 . 460 . 470 . 480 . 490 . 500 . 510 . 520 . 530 . 540 . 550 . 560 . 570 . 580 . 590 . 600 . 610 . 620 . 630 . 640 . 650 . 660 . 670 . 680 . 690 . 700 . 710 . 720 . 730 . 740 . 750 . 760 . 770 . 780 . 790 . 800 . 810 . 820 . 830 . 840 . 850 . 860 . 870 . 880 . 890 . 900 . 910 . 920 . 930 . 940 . 950 . 960 . 970 . 980 . 990 . 1000 . 1010 . 1020 . 1030 . 1040 . 1050 . 1060 . 1070 . 1080 . 1090 . 1100 . 1110

MNDWQWLKNRLVNSKTKSVSFWLPQTSSNIIDIAELIKCCSELKNTSINGLIDFLNQQDKLEFNLTRLKEIDVEDGKQLFGIETSVYKHFQNEIARFYKQVNKHFRETGSESLFLALPVIEGINEFNDIFRAPLLYVGVKLKVSPRFERFWLEINKEEIFLNPTIIGVETNKRNSLFKNNYDTTKIDVNDALKVFSELEYEFRMPLTSELKSFSKKAKSDFNTEKRTNYLINNVLLGIFDVKGDQLFQNFNEILNTDPDVLDELLKDRRDLLLENREFREQFDLKDTYLFSHLDIYQQYAVKQALLGDLIIEGPPGTGKSETIVNILVNLVLNNKKVLFVSEKVTALDVVYNRLGSFKHIALFNASVAAEKKRFYNQFAEFETYFTTYFSKKDLDATLPTFEGKWVDDILGAFQALQALYDTKINSGENLFSFKEIVSSFQMLDASYIKIKEYERFDEWVRVFSNPLWLEKHLSYQELKKELSQRWNGIDNFYQLQSLLNQTKKRKVLNYVLEHFEQFNTVISPKHVLFYKPSNKSQLLLKQLKQDVEQYTSLQRFQSPTKFETIKLNFINQVNENPTPWFFSWFIQFHAKPLLEKLVSFESNIIKTKQAYLNGIESYVASCKKLLKTTILNNFFQLYQTNKDELLEICRQAKNPVLKEITWWFKKHFELLKKLFPVHIMTLESAATLTPNQRGLYDYVVIDEASQVFLERAIPILFRADKYIIAGDTKQLKPANFFQSRAEYDVDEEFEDGNIEAAVHSSSLLHFLKNRSRILTLLKFHYRSDSADLIAFTNNRIYDNELIFMNKANADQRVFIVHDVIDGIWKNNRNLQEARDVVQRLEQLTTTNDYKKSLGVICFNKNQADLIEYLIDKQNNPLLNEWRERQNDVGEYEGLFVKNIENVQGDERDIIIFSLGYDRSVNSYGPISKQGGENRLNVAITRAKQRIELFKTNRGEDYNGLSSSSLGSKLLVEYLLYCEAMAKNQGEKITFQAVKRKETKAKYELAVENDFFNQLQAIFGGEFEIKRNVNEGAYFFSFVFYFNNIPYLAIDFNIPIPTSRKQVMEGILYREQFLKKRQWNLINIWIDEWKLNPIGVISKIRSSLAVHQNQHEEI

tmhmm (0)
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

low complexity (7%)
------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------XXXXXXXXXXXXXXXXXXXXXX------------------------------------------XXXXXXXXXXXXXXXX-----------------------------------------XXXXXXXXXX-------------------------------------------------------------------------------------------------------------------------------------------------XXXXXXXXXXX----------------------------------------------------------------------------------------------------------------------XXXXXXXXXXXX-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------XXXXXXXXXXXX-----------------------------------------------------------------------------------------------------------------------------------------------

coiled-coils (2%)
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------XXXXXXXXXXXXXXXXXXXXX----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

disordered (7%)
---------------------------------------------------XXXXXXXXX-X-X------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------XXXXXXXXXXXX------------------------------------------------------------------------------------------X---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------XXXXXXXXXXXXXXXXXXXXXXX--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------XXXXXXXXXXXXXXXXXXXXX---------------------------------------------------------------------------------------------------------XXXXXX

psipred
--HHHHHHHH----------EE-------HHHHHHHH-------------HHHH------------------HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH---EEEEEEEEEEEEE-----EEEEEEEE--EEEEE------EEEEE----------HHHHHHHHHHHH-----------HHHHHHHHHHHHHH-----HHHHHHHHHHHHHH--------EE---EEEEE----HHHHHHHHHHHHH---HHHHHH-------------------HHH--------HHHHHHHHHHH---EEEE------HHHHHHHHHHHHHH---EEEEE----HHHHHHHHHH------HHH--------HHHHHHHHHHHHHHHHHH----------HHHHH--HHHHHHHHHHHHHHHHH-----------HHH--------------------------------------HHH-------------HHHH---HH--------HHHHHHHHHH----------------------HHHHH-HH---HHHHHHH-----HHHHHHHHHHH--HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH----HHHHHHH---------EEEEE------HHHHHHHHHH---EEE----------EE-HHHHH-----HHHH-HHH------------------HHH--------------------------------------EE-------------HHHHHHHHEEEEEE---------EEEE---HHHHHHHHHHHHH---HHHHHHHHHHH-------EEE------------EEEEEEE------------------EEEEEEE---EEEEEEEE-------------------HHHHHHHHH----------------------------HHHHHHHHHHHH---EEE--------EEEEEEE------EEEEE--------HHHHHHHHHHHHHHHH---EEEEE-HHHHHH-HHHHHHHHHHHHHHHHHHH---

# SignalP-4.0 gram+ predictions
# Measure  Position  Value  Cutoff  signal peptide?
  max. C    30	    0.131
  max. Y    30	    0.166
  max. S    29	    0.331
  mean S     1-29   0.223
       D     1-29   0.189    0.450  NO
Name=tmp_signalp_seq	SP='NO' D=0.189 D-cutoff=0.450 Networks=SignalP-TM

Domain Repeats Prediction

Boundary STD (+/-) Consensus

-- -- --

Ginzu Domain Prediction 1

Domain Span Source Reference Parent Parent Span Confidence Annotations

1-224 alignment 6dfpA_301 1-361 0.169300 --

225-995 alignment 6ej5A_302 1-599 0.394100 --

996-1113 alignment 1vsrA_303 1-134 0.738000 HYDROLASE

Ginzu Domain Prediction 2

Domain Span Source Reference Parent Parent Span Confidence Annotations

1-224 msa 6dfpA_301 1-361 0.169300 --

225-995 alignment 6ej5A_302 1-599 0.394100 --

996-1113 alignment 1vsrA_303 1-134 0.738000 HYDROLASE

PSI-BLAST Sequence Hits (Top 20 by Identity) ALL DATA

TaxID Species Alignments PSI-Blast Data (alignment with lowest E value)

E value Query Span Sbjct Span Bit Score Identity HSP Length Accession Description

662945 Mycoplasma genitalium M6320 1 (0.03%) 0.0 1-1113 1-1113 836 100 1113 ref|NP_072802.1| hypothetical protein MG_140 [Mycoplasma genitalium G37] ref|...

662947 Mycoplasma genitalium M2288 1 (0.03%) 0.0 1-1113 1-1113 836 100 1113 ref|YP_006601161.1| hypothetical protein CM5_00805 [Mycoplasma genitalium M2288]...

662946 Mycoplasma genitalium M6282 1 (0.03%) 0.0 1-1113 1-1113 834 100 1113 ref|YP_006600159.1| hypothetical protein CM3_00880 [Mycoplasma genitalium M6282]...

1238993 Mycoplasma pneumoniae M129-B7 1 (0.03%) 0.0 1-1113 1-1113 776 79 1113 ref|NP_109841.1| hypothetical protein MPN153 [Mycoplasma pneumoniae M129] ref...

710128 Mycoplasma gallisepticum str. R(high) 1 (0.03%) 1E-175 2-1111 3-1125 624 45 1123 ref|NP_852877.2| putative helicase superfamily protein [Mycoplasma gallisepti...

1159204 Mycoplasma gallisepticum NC08_2008.031-4-3P 1 (0.03%) 1E-175 2-1111 3-1125 624 44 1123 ref|YP_006580957.1| helicase superfamily protein [Mycoplasma gallisepticum VA94_...

708616 Mycoplasma gallisepticum str. F 1 (0.03%) 1E-175 2-1111 3-1125 623 44 1123 ref|YP_005880386.1| putative helicase superfamily protein [Mycoplasma gallisepti...

1006581 Mycoplasma gallisepticum S6 1 (0.03%) 1E-175 2-1111 3-1125 623 44 1123 ref|YP_008894037.1| heavy metal transporter CzcA [Mycoplasma gallisepticum S6] r...

670888 Escherichia coli 1827-70 2 (0.06%) 5E-67 679-973 1-296 263 33 303 ref|WP_001339081.1| superfamily I DNA and RNA helicase and helicase subunits-lik...

666686 Bacillus sp. 1NLA3E 1 (0.03%) 1E-56 892-1105 8-234 229 33 230 ref|YP_007910566.1| hypothetical protein B1NLA3E_11585 [Bacillus sp. 1NLA3E] ref...

1246476 Arthrobacter crystallopoietes BAB-32 1 (0.03%) 1E-112 679-1100 1-414 412 29 436 ref|WP_005266179.1| superfamily I DNA and RNA helicase protein [Arthrobacter cry...

320771 bacterium Ellin514 1 (0.03%) 1E-61 642-954 1221-1564 246 29 348 ref|WP_007416627.1| DNA helicase [Pedosphaera parvula] gb|EEF59341.1| conserved ...

690850 Desulfovibrio africanus str. Walvis Bay 1 (0.03%) 1E-112 653-1108 1241-1686 415 28 467 ref|YP_005053400.1| hypothetical protein [Desulfovibrio africanus str. Walvis Ba...

293091 Haloquadratum walsbyi 1 (0.03%) 1E-110 638-1108 27-488 405 28 485 ref|WP_021051661.1| protein of unknown function (DUF559), partial [Haloquadratum...

1116126 Escherichia coli 2846750 1 (0.03%) 1E-109 683-1113 1-423 402 28 442 ref|WP_001705641.1| AAA domain protein [Escherichia coli] gb|EMV50957.1| AAA dom...

562 Escherichia coli 3 (0.09%) 1E-105 696-1113 3-412 390 28 429 ref|WP_024173159.1| hypothetical protein [Escherichia coli]

1262456 Ralstonia solanacearum FQY_4 1 (0.03%) 1E-119 652-1113 1198-1657 436 27 477 ref|YP_008237658.1| DNA helicase related protein [Ralstonia solanacearum FQY_4] ...

296591 Polaromonas sp. JS666 2 (0.06%) 1E-119 653-1107 1521-1972 435 27 467 ref|YP_548781.1| superfamily I DNA/RNA helicase [Polaromonas sp. JS666] ref|W...

48292 Hyphomicrobium zavarzinii 2 (0.06%) 1E-117 609-1110 1244-1735 430 27 512 ref|WP_020088070.1| hypothetical protein [Hyphomicrobium zavarzinii]

446469 Sanguibacter keddieii DSM 10542 1 (0.03%) 1E-115 610-1111 1124-1615 423 27 515 ref|YP_003315152.1| hypothetical protein Sked_24040 [Sanguibacter keddieii DSM 1...