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Fragment Libraries			Alanine Scanning			DNA Interface Scan
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Job 88379 [SSGCID - MygeA.20186.a] [2019-01-18] [D-172-16-30-137.dhcp4.x.x] [Structure] [Complete] DNA polymerase III PolC-type (MG_031) - BATCH:88256

Features and Secondary Structure

1 . 10 . 20 . 30 . 40 . 50 . 60 . 70 . 80 . 90 . 100 . 110 . 120 . 130 . 140 . 150 . 160 . 170 . 180 . 190 . 200 . 210 . 220 . 230 . 240 . 250 . 260 . 270 . 280 . 290 . 300 . 310 . 320 . 330 . 340 . 350 . 360 . 370 . 380 . 390 . 400 . 410 . 420 . 430 . 440 . 450 . 460 . 470 . 480 . 490 . 500 . 510 . 520 . 530 . 540 . 550 . 560 . 570 . 580 . 590 . 600 . 610 . 620 . 630 . 640 . 650 . 660 . 670 . 680 . 690 . 700 . 710 . 720 . 730 . 740 . 750 . 760 . 770 . 780 . 790 . 800 . 810 . 820 . 830 . 840 . 850 . 860 . 870 . 880 . 890 . 900 . 910 . 920 . 930 . 940 . 950 . 960 . 970 . 980 . 990 . 1000 . 1010 . 1020 . 1030 . 1040 . 1050 . 1060 . 1070 . 1080 . 1090 . 1100 . 1110 . 1120 . 1130 . 1140 . 1150 . 1160 . 1170 . 1180 . 1190 . 1200 . 1210 . 1220 . 1230 . 1240 . 1250 . 1260 . 1270 . 1280 . 1290 . 1300 . 1310 . 1320 . 1330 . 1340 . 1350 . 1360 . 1370 . 1380 . 1390 . 1400 . 1410 . 1420 . 1430 . 1440 . 1450

MVFNLENEKDKKVLALSNFLIDNNILDHNELNSLIERIESIYFNKYIEEKVFLFAITISRPLTIDIWNALYEGFNELNEGFKADNESFKLTITFKENEPFFKSKNSFSSVTIAIIKDYFHSFFSKDKKYKILIEQELSNPNFLSYENDELKAQCQTKELTEWLVQKSKSFIFWMNNAGFKNFNFIALYPIDKNESKLKVKAVTVSQYDKQFETKVFATEFIPIHKINQQIDDVKIIGQIFELKTHESLTGKKTLNIYVTDFQLGGSLILKWSYTDEKKIEGITIGNWIKAHIQVERDPNTQILYGIVREINPVEIPNNYKRLDLSKQKRVELVFHTKMTAFDGINDIEEYAQFAKERGWKAITVTDKDNIHIYPKFYEVAKKYDLKAIYGLEFNLTDDHIKIVHNPDNTKLSDATFVIFDIETTGLHGRYDDVIEFSARKIKNNSEIDHQQFFLKIDKPIPKTITEITKITDEMLEGGIDQQQGLEKIRNYLDDCVMVAHNGINFDLPFLQTQFEKYNIKPLTNPLIDTLCLSWALNPLFSSHTLSNICSKLKLEFDDERLHRAEYDTEALKKVFFYFKKQLKEMGINTLTEIDQNLNKKCQIDLMKRVFTNTAIVYVKNQRGFQNLYEMLSIALTDHNANRPLVLASSLAKFRKSFLLTENPVQGDIFKAALTKPINELEKAIEKVDFVLISQPNAYLGYTLREGLKKELINDAIKLVIKTATKLKKLVAVASDAYFIHPWENEYYKAIVCAKGLGGKWHRHFNNKEKEQTVPEVFLHTTDEMLKRMSFLGEDIAYKLVVENTNKIVKLLDLNELVPTKNKLYPPVMQDSNQKLIDKTWKQAEKRYGKNLPKLIKERIEKELNAIISNGFGIVFWISHLLVEQSVKDGYFVGPRGSIGSSLIANLIGISEINPLAAHYLCEQCHYFEVSDSVDDGYDLMIRDCPKCHEKASFKGDGHNIPFATFMGFSGDKIPDIDLNFSSEYQAKAHDYVRKLFGVNNTFRAGTIATVAEKTAYGYARNYFEIIKRVDLATTAEIERFKQKLIGIKRTTGQHPGGIMIFPSDHSVYEFTPCGFPADDVESEWKTTHFEYDALGDAILKLDILGQDDPTMLKHLADLTKINPQNIPHFDKNLISMFSSNKPLNLKPGIVDEVTGAVGIPEFGTKFVRKILEQTKPKDFADLIRVSGLSHGKNVWADNAQKLIKSNRLTLRDVIACRDDIMLYLINKGMQAKDAFEIMEKVRKGIKVNAKEVSLMQNCGVEQYWINACLKINYLFPKAHAAAYVLMAWRIAWFKLYHPLSYYACLLSFKLKEHDINGFEKGYEFIKNRLDELNKLYRIKKIKPKEAELLTSYEVYLEMMARNIKLQQISIQNSNARMFVEHNGVLIAPFITIPGMGEAVASSIVEARNEKPFASLNDFKKRTKITKKHVETMEQLQLFDEFEHQDDHKLFN

tmhmm (0)
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

low complexity (1%)
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------XXXXXXXXXXXXXXXXXXX------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

coiled-coils (0%)
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

disordered (5%)
XXXXXXXXXXXXXX---------------------------------------------------------------------------------------------------------------------------------------------------------------XXX-XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX------------------------------------------------------------------------------------------------XXXXXXXXXXXXXXX------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------XXXXX--------------------------------------------------------------------------------------------------------------

psipred
----HHHHHHHHHHHHHHHHHHHHH--HHHHHHHHHHEEEEEEEEE-EEEEEEEEE-------HHHHHHHHHHHHHHHHHHHHHH--EEEEEEHHH--HHHHHHHHH-HHHHHHHHHHHHHHHHHHH--HHHHHHHHHHHHHHHH---HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH--HHHHHH-------------------------EE-HHHHH----EEEEEEEEEEEEEEE-----EEEEEEEE------EEEEEEE---HHHHH------EEEEEEEEEE-----EEEEEEEEEEEE---------------EEEEEE------------HHHHHHHHHH----EEEEE-----EEHHHHHHHHHH------------------------------HHHHHHHHHH----------EEEEEEEEEE--EEEEEEEEEE-------HHHHHH----HHHHH----HHHHHHHHHHHH---EEEE----HHHHHHHHHHHHHH--------EEEEEHHHHHH--------HHHHHHHH------HHHHHHHHHHHHHHHHHHH-------------------------------------------------HHHHHHHHHHH-------HHHHHHHHH-----EE-------HHHHHH--HHHHHHHHHHHHHHHHH--HHHHHHHHH-----HHHHHHHHHHHHHHHHHH---------EEEE-HHHHHHHHHHHHHH------------HHHH---HHHH--HHHHHHHHHHH--HHHHHHHHHHHHHHHHHH--------------------HHHHHHHHHHHHHHHH------HHHHHHHHHHHHHHHH---HHHHHHHHHHHHHHHH-----------HHHHHHHHH-------------------HHH----------------HHHHHHHHH---EEEEEEEE--HHHHHHHHHH--------HHHHHHHHHH----HHHHHHHHHHHHHHHHHHHHHHHHHHHH----HHHHHHHHHHHHH------------EEEE-------------EE---------EEE---HHHHHH------------HHHHHHHHHHH---HH------HHHHHHH---------HHHHH-----EEE-----HHHHHHHHH-----HHHHHHHHHH------------------------------HHHHHHHHHHHHHHHHHHHHHHHHHHHHH---HH---HH---HHHHHHH----------HHHHHHHHHHHHHHHHHH--HHHHHHHHH-------HHHHHHHHHHHHHHHHHHHHHHH------------HHHHHHHHHHHH----------------EEE----EEEEEHHH-----HHHHHHHHHHH-------HHHHHHHH---HHHHHHHHH--------HHHHHH---

# SignalP-4.0 gram+ predictions
# Measure  Position  Value  Cutoff  signal peptide?
  max. C    70	    0.111
  max. Y     5	    0.146
  max. S     4	    0.237
  mean S     1-4    0.207
       D     1-4    0.169    0.450  NO
Name=tmp_signalp_seq	SP='NO' D=0.169 D-cutoff=0.450 Networks=SignalP-TM

Domain Repeats Prediction

Boundary STD (+/-) Consensus

-- -- --

Ginzu Domain Prediction 1

Domain Span Source Reference Parent Parent Span Confidence Annotations

1-214 alignment 5g1rF_301 1-192 0.1416 --

215-1451 alignment 3f2cA_203 1-993 0.652800 TRANSFERASE/DNA

Ginzu Domain Prediction 2

Domain Span Source Reference Parent Parent Span Confidence Annotations

1-214 cutpref -- -- 0.000000 --

215-1451 alignment 3f2cA_203 1-993 0.652800 TRANSFERASE/DNA

PSI-BLAST Sequence Hits (Top 20 by Identity) ALL DATA

TaxID Species Alignments PSI-Blast Data (alignment with lowest E value)

E value Query Span Sbjct Span Bit Score Identity HSP Length Accession Description

663918 Mycoplasma genitalium M2321 1 (0.03%) 0.0 1-1451 1-1451 1304 100 1451 ref|NP_072691.1| DNA polymerase III PolC [Mycoplasma genitalium G37] ref|YP_0...

662946 Mycoplasma genitalium M6282 1 (0.03%) 0.0 1-1451 1-1451 1302 100 1451 ref|YP_006600043.1| DNA polymerase III PolC [Mycoplasma genitalium M6282] ref|WP...

662945 Mycoplasma genitalium M6320 1 (0.03%) 0.0 1-1451 1-1451 1302 100 1451 ref|YP_006600531.1| DNA polymerase III PolC [Mycoplasma genitalium M6320] ref|WP...

662947 Mycoplasma genitalium M2288 1 (0.03%) 0.0 1-1451 1-1451 1300 100 1451 ref|YP_006601040.1| DNA polymerase III PolC [Mycoplasma genitalium M2288] ref|WP...

1238993 Mycoplasma pneumoniae M129-B7 1 (0.03%) 0.0 1-1450 1-1443 1222 73 1452 ref|YP_007347664.1| DNA polymerase III, alpha subunit (gram-positive type) [Myco...

272634 Mycoplasma pneumoniae M129 1 (0.03%) 0.0 1-1450 1-1443 1221 73 1452 ref|NP_109722.1| DNA polymerase III PolC [Mycoplasma pneumoniae M129] ref|WP_...

1112856 Mycoplasma pneumoniae 309 1 (0.03%) 0.0 1-1450 1-1443 1220 73 1452 ref|YP_005175266.1| DNA polymerase III subunit alpha [Mycoplasma pneumoniae 309]...

722438 Mycoplasma pneumoniae FH 1 (0.03%) 0.0 1-1450 1-1443 1220 73 1452 ref|YP_005881079.1| DNA polymerase III subunit alpha [Mycoplasma pneumoniae FH] ...

1249 Weissella paramesenteroides 1 (0.03%) 1E-32 1161-1282 1-122 149 49 122 gb|AAV48975.1| polC [Weissella paramesenteroides]

29397 Lactobacillus delbrueckii subsp. lactis 1 (0.03%) 6E-28 1073-1162 1-90 134 46 90 gb|AAQ07150.1|AF496460_1 DNA polymerase III, alpha chain [Lactobacillus delbrueckii s...

879300 Lactobacillus iners LactinV 11V1-d 1 (0.03%) 0.0 774-1450 2-674 819 44 681 ref|WP_006730931.1| DNA polymerase III subunit alpha [Lactobacillus iners] gb|EF...

879299 Lactobacillus iners LactinV 09V1-c 1 (0.03%) 0.0 807-1450 2-641 780 44 648 ref|WP_006731816.1| DNA polymerase III subunit alpha [Lactobacillus iners] gb|EF...

1138874 Streptococcus pyogenes NS88.2 1 (0.03%) 0.0 784-1451 1-670 799 43 676 ref|WP_002994568.1| DNA polymerase III subunit alpha [Streptococcus pyogenes] em...

1359 Lactococcus lactis subsp. cremoris 1 (0.03%) 3E-88 1178-1451 1-278 334 40 280 emb|CAA75320.1| DNA polymerase III alpha chain-like protein [Lactococcus lac...

401526 Thermosinus carboxydivorans Nor1 2 (0.05%) 0.0 613-1450 398-1220 950 39 842 ref|WP_007288397.1| DNA polymerase III subunit alpha [Thermosinus carboxydivoran...

1149859 Pelosinus fermentans A12 1 (0.03%) 0.0 613-1450 60-882 919 39 842 ref|WP_007949697.1| DNA polymerase III polC-type, partial [Pelosinus fermentans]...

1149861 Pelosinus fermentans B3 1 (0.03%) 0.0 613-1450 398-1220 917 39 842 ref|WP_007934203.1| DNA polymerase III subunit alpha [Pelosinus] gb|EIW18528.1| ...

1192197 Pelosinus fermentans JBW45 1 (0.03%) 0.0 613-1450 398-1220 915 39 842 ref|WP_007956388.1| DNA polymerase III subunit alpha [Pelosinus fermentans] gb|E...

645991 Syntrophobotulus glycolicus DSM 8271 1 (0.03%) 0.0 597-1450 410-1250 915 39 860 ref|YP_004266546.1| DNA polymerase III subunit alpha [Syntrophobotulus glycolicu...

699218 Megasphaera genomosp. type_1 str. 28L 2 (0.05%) 0.0 612-1450 362-1197 890 39 854 ref|WP_009369957.1| DNA polymerase III subunit alpha [Megasphaera genomosp. type...